pRK5 FLAG DEPTOR (13 S/T->A) | Addgene 21702 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pRK5 FLAG DEPTOR (13 S/T->A)

catalog :

21702

citations: 3

Reference
Csukasi F, Bosakova M, Bárta T, Martin J, Arcedo J, Barad M, et al. Skeletal diseases caused by mutations in PTH1R show aberrant differentiation of skeletal progenitors due to dysregulation of DEPTOR. Front Cell Dev Biol. 2022;10:963389 pubmed publisher
GonzÃ¡lez TerÃ¡n B, LÃ³pez J, RodrÃguez E, Leiva L, MartÃnez MartÃnez S, Bernal J, et al. p38Î³ and Î´ promote heart hypertrophy by targeting the mTOR-inhibitory protein DEPTOR for degradation. Nat Commun. 2016;7:10477 pubmed publisher
Peterson T, Laplante M, Thoreen C, Sancak Y, Kang S, Kuehl W, et al. DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for their survival. Cell. 2009;137:873-86 pubmed publisher

product information

Catalog Number :

21702

Product Name :

pRK5 FLAG DEPTOR (13 S/T->A)

article :

doi	10.1016/j.cell.2009.03.046
id	2654
pubmed_id	19446321

bacterial resistance :

Ampicillin

cloning :

backbone

pRK5-FLAG

backbone_mutation

backbone_origin

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

DEPTOR phosphorylation sites depicted in Author's Map link.

origin :

pi :

alt_names

DEPDC6

DEPTOR

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	SalI
promoter
sequencing_primer_3
sequencing_primer_5	SP6
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	DEP.6, DEPDC6
gene	DEPTOR
id	64798

genbank_ids

NM_022783

mutation

Nonphosphorylatable mutant. 13xA. Also has N204S and S389N mutations (numbering according to associated publication)

name

DEPTOR 13xA

shRNA_sequence

size

2350

species

9606

Homo sapiens