pJF6 | Addgene 21678 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pJF6

catalog :

21678

citations: 1

Reference
Fishman Lobell J, Rudin N, Haber J. Two alternative pathways of double-strand break repair that are kinetically separable and independently modulated. Mol Cell Biol. 1992;12:1292-303 pubmed

product information

Catalog Number :

21678

Product Name :

pJF6

article :

doi
id	2723
pubmed_id	1545810

bacterial resistance :

Ampicillin

cloning :

backbone

pJH271

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

centromeric plasmid

growth notes :

The HindIII fragment of pJF3 containing URA3 UAScycl- and 300 bp of the LEU2 gene promoter and LacZ::CS cut site was inserted into the HindIII site at the upstream end of a promotorless LacZ gene on plasmid JH271. The two LacZ sequences are in direct repeat orientation relative to each other. The lambda sequences are of unknown origin Please note: Addgene was not able to sequence verify the critical features of this plasmid due to its repetitive nature.

origin :

pi :

alt_names

2 direct repeat .copies of lacZ

see author's map

cloning

clone_method	Restriction Enzyme
cloning_site_3	Hind III
cloning_site_5	Hind III
promoter
sequencing_primer_3
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ECIAI39_0334
gene	lacZ
id	7151913

genbank_ids

mutation

HO digestion site in one of the LacZ genes

name

LacZ

shRNA_sequence

size

species

E. coli