This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pNR6
catalog :
21677
citations: 1
Reference
Rudin N, Haber J. Efficient repair of HO-induced chromosomal breaks in Saccharomyces cerevisiae by recombination between flanking homologous sequences. Mol Cell Biol. 1988;8:3918-28 pubmed
product information
Catalog Number :
21677
Product Name :
pNR6
article :
doi
id2722
pubmed_id3065627
bacterial resistance :
Ampicillin
cloning :
backbonepRK113
backbone_mutation
backbone_originR. Kostriken
backbone_size2700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
integration sequence
growth notes :
Constructed by inserting a HindIII URA3 fragment into the HindIII site of the multiple cloning site of plasmid pRK113. Contains the 117-bp HO cut site of MATa inserted between the HincII and BamHI sites of pUC9 and the URA3 1.17-kb HindIII fragment at the HindlIl site transcribed away from the HO cut site.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Hind III
cloning_site_5Hind III
promoter
sequencing_primer_3M13-reverse
sequencing_primer_5M13-forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYEL021W
geneURA3
id856692
genbank_ids
mutation
nameUra3
shRNA_sequence
size1162
species
4932
Saccharomyces cerevisiae
tags
resistance markers :
183
tags :
Unknown
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA