pIS1 DICER1 long-mut-all | Addgene 21653 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pIS1 DICER1 long-mut-all

catalog :

21653

citations: 2

Reference
Yang C, Li H, Ku T, Wu C, Sim K, Lo S. MicroRNA-Independent Modulation of DICER1 Expression by hAgo2. Mol Cell Biol. 2020;40: pubmed publisher
Mayr C, Bartel D. Widespread shortening of 3'UTRs by alternative cleavage and polyadenylation activates oncogenes in cancer cells. Cell. 2009;138:673-84 pubmed publisher

product information

Catalog Number :

21653

Product Name :

pIS1 DICER1 long-mut-all

article :

doi	10.1016/j.cell.2009.06.016
id	2868
pubmed_id	19703394

bacterial resistance :

Ampicillin

cloning :

backbone

pIS1

backbone_mutation

backbone_origin

Available from Addgene (#12179)

backbone_size

4085

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

growth notes :

Long means that all functional alternative polyadenylation signals have been deleted. Mut means that all microRNA binding sites for an indicated microRNA have been mutated.

origin :

pi :

alt_names

CM12

cloning

clone_method	Restriction Enzyme
cloning_site_3	Not I
cloning_site_5	Xba I
promoter
sequencing_primer_3	EBV Reverse
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2, aviD
gene	DICER1
id	23405

genbank_ids

mutation

Mutated let-7 and miR103/107 microRNA binding sites

name

Dicer 1

shRNA_sequence

size

4200

species

9606

Homo sapiens