This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV5-FLAG-Gene33
catalog :
21633
citations: 1
Reference
Makkinje A, Quinn D, Chen A, Cadilla C, Force T, Bonventre J, et al. Gene 33/Mig-6, a transcriptionally inducible adapter protein that binds GTP-Cdc42 and activates SAPK/JNK. A potential marker transcript for chronic pathologic conditions, such as diabetic nephropathy. Possible role in the response to persistent stres. J Biol Chem. 2000;275:17838-47 pubmed
product information
Catalog Number :
21633
Product Name :
pCMV5-FLAG-Gene33
article :
doi10.1074/jbc.M909735199
id2702
pubmed_id10749885
bacterial resistance :
Ampicillin
cloning :
backbonepCMV5
backbone_mutation
backbone_originDr. David Russell, UTSW
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Plasmid was also used in Xu et al. 2005 the construct can be used in cardiomyocytes There is a V to L amino acid change at position 6. The lab feels it is conservative enough not to matter.
origin :
37
pi :
alt_names
Mig6, Ralt, ERRFI
cloning
clone_methodRestriction Enzyme
cloning_site_3Sal I
cloning_site_5Eco RI
promoter
sequencing_primer_3
sequencing_primer_5CMV Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesRGD1307599, Ralt, Xxx, gene 33
geneErrfi1
id313729
genbank_ids
x07266
mutation
nameGene33
shRNA_sequence
size1380
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagFLAG
resistance markers :
269
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA