This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pKP332 (Lenti-OSK1)
catalog :
21627
citations: 5
Reference
Cheng J, Park D, Berrios C, White E, Arora R, Yoon R, et al. Merkel cell polyomavirus recruits MYCL to the EP400 complex to promote oncogenesis. PLoS Pathog. 2017;13:e1006668 pubmed publisher
Kim S, Oceguera Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, et al. KLF4 N-terminal variance modulates induced reprogramming to pluripotency. Stem Cell Reports. 2015;4:727-43 pubmed publisher
Lu H, Chai C, Lim T, Leong M, Lim J, Gao S, et al. A defined xeno-free and feeder-free culture system for the derivation, expansion and direct differentiation of transgene-free patient-specific induced pluripotent stem cells. Biomaterials. 2014;35:2816-26 pubmed publisher
Bedel A, Taillepierre M, Guyonnet Duperat V, Lippert E, Dubus P, Dabernat S, et al. Metabolic correction of congenital erythropoietic porphyria with iPSCs free of reprogramming factors. Am J Hum Genet. 2012;91:109-21 pubmed publisher
Chang C, Lai Y, Pawlik K, Liu K, Sun C, Li C, et al. Polycistronic lentiviral vector for "hit and run" reprogramming of adult skin fibroblasts to induced pluripotent stem cells. Stem Cells. 2009;27:1042-9 pubmed publisher
product information
Catalog Number :
21627
Product Name :
pKP332 (Lenti-OSK1)
article :
doi10.1002/stem.39
id2709
pubmed_id19415770
bacterial resistance :
Ampicillin
cloning :
backbonepDL171 (pWPI with deletion of 1409 bp XhoI [EMCV IRES GFP] frag)
backbone_mutation
backbone_origin
backbone_size9692
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
Cre/Lox
growth notes :
Plasmid constructed by Kevin Pawlik
origin :
37
pi :
alt_names
POU5F1
Porcine teschovirus-1 2A peptide
cloning
clone_methodRestriction Enzyme
cloning_site_3SwaI
cloning_site_5SwaI
promoter
sequencing_primer_3AATCCAGAGGTTGATTATCG
sequencing_primer_5GGCACCTCGATTAGTTCTCGAGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesOCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
genePOU5F1
id5460
aliasesANOP3, MCOPS3
geneSOX2
id6657
aliasesEZF, GKLF
geneKLF4
id9314
genbank_ids
BC117435
BC029923
BC013923
mutation
namehOCT4 - 2A - hSOX2 - 2A - hKLF4
shRNA_sequence
size3589
species
9606
Homo sapiens
32644
Other
Porcine teschovirus-1
tags
resistance markers :
531
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA