This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTOPO-MCL1S159A
catalog :
21606
citations: 3
Reference
Sena E, Bou Rouphael J, Rocques N, Carron Homo C, Durand B. Mcl1 protein levels and Caspase-7 executioner protease control axial organizer cells survival. Dev Dyn. 2020;249:847-866 pubmed publisher
Bailey M, Singh T, Mero P, Moffat J, Hieter P. Dependence of Human Colorectal Cells Lacking the FBW7 Tumor Suppressor on the Spindle Assembly Checkpoint. Genetics. 2015;201:885-95 pubmed publisher
Maurer U, Charvet C, Wagman A, Dejardin E, Green D. Glycogen synthase kinase-3 regulates mitochondrial outer membrane permeabilization and apoptosis by destabilization of MCL-1. Mol Cell. 2006;21:749-60 pubmed
product information
Catalog Number :
21606
Product Name :
pTOPO-MCL1S159A
article :
doi10.1016/j.molcel.2006.02.009
id2704
pubmed_id16543145
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1 /V5-His-TOPO
backbone_mutation
backbone_originInvitrogen
backbone_size5523
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
BCL2L3
NM_021960
cloning
clone_methodTOPO Cloning
cloning_site_3T/A
cloning_site_5T/A
promoter
sequencing_primer_3BGH reverse
sequencing_primer_5T7
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesBCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT
geneMCL1
id4170
genbank_ids
NM_021960
mutationSerine 159 mutated to Alanine, abolishing phosphorylation by GSK-3. Insert ends with a STOP codon, no V5-His-tag.
nameMCL-1 S159A
shRNA_sequence
size1052
species
9606
Homo sapiens
tags
resistance markers :
318
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA