This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
punc-119c
catalog :
21595
citations: 1
Reference
Ferguson A, Fisher A. Retrofitting ampicillin resistant vectors by recombination for use in generating C. elegans transgenic animals by bombardment. Plasmid. 2009;62:140-5 pubmed publisher
product information
Catalog Number :
21595
Product Name :
punc-119c
article :
doi10.1016/j.plasmid.2009.06.001
id2699
pubmed_id19520111
bacterial resistance :
Kanamycin
cloning :
backboneR6K origin PCR fragment
backbone_mutation
backbone_originEpicentre
backbone_size300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Worm Expression
Other
Adds unc-119 marker to Amp resistant plasmids
growth notes :
This plasmid has small deletions within the KanR gene. We generated these fragments by PCR so it is definitely possible that these were generated during amplification, these changes must not impair function as the construct is Kan resistant both as a plasmid and following recombination.
growth temp :
Requires pir expressing bacterial strain such as EC100 pir-116
inserts :
Pir116
origin :
37
pi :
alt_names
kanamycin resistance
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHi
cloning_site_5BamHI
promoter
sequencing_primer_3GGTCTGACAGTTACCAATGCTTAATC
sequencing_primer_5GCACTTTTCGGGGAAATGTGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_M142.1
geneunc-119
id176519
genbank_ids
NM_066998
mutationN/A
nameunc-119
shRNA_sequence
size3200
species
6239
Caenorhabditis elegans
tags
plasmid copy :
The R6K replication origin was generated via PCR from pMOD4 (Epicentre). The kanamycin resistance gene was generated via PCR from pKRP11 (The Cloning Vector collection). The unc-119 gene was subcloned from pCG150 (available from Addgene).
resistance markers :
148
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA