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product summary
company name :
Addgene
product type :
cDNA
product name :
pCDNA3-Myc3-Nrf2
catalog :
21555
citations: 29
Reference
Htut N, Onkoksoong T, Saelim M, Kueanjinda P, Sampattavanich S, Panich U. Live-cell imaging Unveils stimulus-specific dynamics of Nrf2 activation in UV-exposed melanoma cells: Implications for antioxidant compound screening. Free Radic Biol Med. 2024;211:1-11 pubmed publisher
Aboulkassim T, Tian X, Liu Q, Qiu D, Hancock M, Wu J, et al. A NRF2 inhibitor selectively sensitizes KEAP1 mutant tumor cells to cisplatin and gefitinib by restoring NRF2-inhibitory function of KEAP1 mutants. Cell Rep. 2023;42:113104 pubmed publisher
Dong L, Xu M, Li Y, Xu W, Wu C, Zheng H, et al. SMURF1 attenuates endoplasmic reticulum stress by promoting the degradation of KEAP1 to activate NRF2 antioxidant pathway. Cell Death Dis. 2023;14:361 pubmed publisher
Xia Q, Li Y, Xu W, Wu C, Zheng H, Liu L, et al. Enhanced liquidity of p62 droplets mediated by Smurf1 links Nrf2 activation and autophagy. Cell Biosci. 2023;13:37 pubmed publisher
Fang X, Lee Y, Jang J, Kim S, Kim S, Kim D, et al. ARD1 stabilizes NRF2 through direct interaction and promotes colon cancer progression. Life Sci. 2023;313:121217 pubmed publisher
Hertel A, Alves L, Dutz H, Tascher G, Bonn F, Kaulich M, et al. USP32-regulated LAMTOR1 ubiquitination impacts mTORC1 activation and autophagy induction. Cell Rep. 2022;41:111653 pubmed publisher
Van Hall Beauvais A, Poganik J, Huang K, Parvez S, Zhao Y, Lin H, et al. Z-REX uncovers a bifurcation in function of Keap1 paralogs. elife. 2022;11: pubmed publisher
Zhang S, Wang J, Wang L, Aliyari S, Cheng G. SARS-CoV-2 virus NSP14 Impairs NRF2/HMOX1 activation by targeting Sirtuin 1. Cell Mol Immunol. 2022;: pubmed publisher
Castiglione G, Xu Z, Zhou L, Duh E. Adaptation of the master antioxidant response connects metabolism, lifespan and feather development pathways in birds. Nat Commun. 2020;11:2476 pubmed publisher
Ong A, Saeidi S, Chi N, Kim S, Kim D, Kim S, et al. The positive feedback loop between Nrf2 and phosphogluconate dehydrogenase stimulates proliferation and clonogenicity of human hepatoma cells. Free Radic Res. 2020;:1-12 pubmed publisher
Shin J, Chun K, Kim D, Kim S, Kim S, Cho N, et al. Curcumin induces stabilization of Nrf2 protein through Keap1 cysteine modification. Biochem Pharmacol. 2020;173:113820 pubmed publisher
Liu N, Lin X, Huang C. Activation of the reverse transsulfuration pathway through NRF2/CBS confers erastin-induced ferroptosis resistance. Br J Cancer. 2020;122:279-292 pubmed publisher
Haley J, Ruiz C, Montal E, Wang D, Haley J, Girnun G. Decoupling of Nrf2 Expression Promotes Mesenchymal State Maintenance in Non-Small Cell Lung Cancer. Cancers (Basel). 2019;11: pubmed publisher
Guo H, Xu J, Zheng Q, He J, Zhou W, Wang K, et al. NRF2 SUMOylation promotes de novo serine synthesis and maintains HCC tumorigenesis. Cancer Lett. 2019;466:39-48 pubmed publisher
Czaniecki C, Ryan T, Stykel M, Drolet J, Heide J, Hallam R, et al. Axonal pathology in hPSC-based models of Parkinson's disease results from loss of Nrf2 transcriptional activity at the Map1b gene locus. Proc Natl Acad Sci U S A. 2019;: pubmed publisher
Wang X, Gai Y, Li B, Huang L. Andalucin from Artemisia lannta suppresses the neuroinflammation via the promotion of Nrf2-mediated HO-1 levels by blocking the p65-p300 interaction in LPS-activated BV2 microglia. Phytomedicine. 2018;51:226-232 pubmed publisher
Kerins M, Ooi A. A catalogue of somatic NRF2 gain-of-function mutations in cancer. Sci Rep. 2018;8:12846 pubmed publisher
Fu Y, Long M, Wisitpitthaya S, Inayat H, Pierpont T, Elsaid I, et al. Nuclear RNR-α antagonizes cell proliferation by directly inhibiting ZRANB3. Nat Chem Biol. 2018;14:943-954 pubmed publisher
Jena K, Kolapalli S, Mehto S, Nath P, Das B, Sahoo P, et al. TRIM16 controls assembly and degradation of protein aggregates by modulating the p62-NRF2 axis and autophagy. EMBO J. 2018;37: pubmed publisher
Zhu B, Tang L, Chen S, Yin C, Peng S, Li X, et al. Targeting the upstream transcriptional activator of PD-L1 as an alternative strategy in melanoma therapy. Oncogene. 2018;37:4941-4954 pubmed publisher
Ebelt N, Kaoud T, Edupuganti R, Van Ravenstein S, Dalby K, Van Den Berg C. A c-Jun N-terminal kinase inhibitor, JNK-IN-8, sensitizes triple negative breast cancer cells to lapatinib. Oncotarget. 2017;8:104894-104912 pubmed publisher
Lacher S, Alazizi A, Wang X, Bell D, Pique Regi R, Luca F, et al. A hypermorphic antioxidant response element is associated with increased MS4A6A expression and Alzheimer's disease. Redox Biol. 2018;14:686-693 pubmed publisher
Ge W, Zhao K, Wang X, Li H, Yu M, He M, et al. iASPP Is an Antioxidative Factor and Drives Cancer Growth and Drug Resistance by Competing with Nrf2 for Keap1 Binding. Cancer Cell. 2017;32:561-573.e6 pubmed publisher
Long M, Lin H, Parvez S, Zhao Y, Poganik J, Huang P, et al. β-TrCP1 Is a Vacillatory Regulator of Wnt Signaling. Cell Chem Biol. 2017;24:944-957.e7 pubmed publisher
Sabouny R, Fraunberger E, Geoffrion M, Ng A, Baird S, Screaton R, et al. The Keap1-Nrf2 Stress Response Pathway Promotes Mitochondrial Hyperfusion Through Degradation of the Mitochondrial Fission Protein Drp1. Antioxid Redox Signal. 2017;27:1447-1459 pubmed publisher
Parvez S, Long M, Lin H, Zhao Y, Haegele J, Pham V, et al. T-REX on-demand redox targeting in live cells. Nat Protoc. 2016;11:2328-2356 pubmed publisher
Cagalinec M, Liiv M, Hodurova Z, Hickey M, Vaarmann A, Mandel M, et al. Role of Mitochondrial Dynamics in Neuronal Development: Mechanism for Wolfram Syndrome. PLoS Biol. 2016;14:e1002511 pubmed publisher
Syu J, Chi J, Kung H. Nrf2 is the key to chemotherapy resistance in MCF7 breast cancer cells under hypoxia. Oncotarget. 2016;7:14659-72 pubmed publisher
Furukawa M, Xiong Y. BTB protein Keap1 targets antioxidant transcription factor Nrf2 for ubiquitination by the Cullin 3-Roc1 ligase. Mol Cell Biol. 2005;25:162-71 pubmed
product information
Catalog Number :
21555
Product Name :
pCDNA3-Myc3-Nrf2
article :
doi10.1128/MCB.25.1.162-171.2005
id2686
pubmed_id15601839
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5437
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Mammalian expression of human Nrf2 with Myc tag
origin :
37
pi :
alt_names
NFE2L2
NRF2
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5BamHI
promoter
sequencing_primer_3SP6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHEBP1, IMDDHH, NRF2, Nrf-2
geneNFE2L2
id4780
genbank_ids
Q16236
mutation
namenuclear factor (erythroid-derived 2)-like 2
shRNA_sequence
size1825
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagmyc3
resistance markers :
593
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA