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product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-EGFP-C4-Nrf2
catalog :
21549
citations: 25
Reference
Liu Y, Chou F, Lang F, Zhang M, Song H, Zhang W, et al. Protein Kinase B (PKB/AKT) Protects IDH-Mutated Glioma from Ferroptosis via Nrf2. Clin Cancer Res. 2023;29:1305-1316 pubmed publisher
Dutta A, Das A, Bisht D, Arya V, Muthuswami R. PLK-1 Interacting Checkpoint Helicase, PICH, Mediates Cellular Oxidative Stress Response. Epigenomes. 2022;6: pubmed publisher
Gopallawa I, Kuek L, Adappa N, Palmer J, Lee R. Small-molecule Akt-activation in airway cells induces NO production and reduces IL-8 transcription through Nrf-2. Respir Res. 2021;22:267 pubmed publisher
Waghela B, Vaidya F, Pathak C. Upregulation of NOX-2 and Nrf-2 Promotes 5-Fluorouracil Resistance of Human Colon Carcinoma (HCT-116) Cells. Biochemistry (Mosc). 2021;86:262-274 pubmed publisher
Zhang W, Cheng C, Sha Z, Chen C, Yu C, Lv N, et al. Rosmarinic acid prevents refractory bacterial pneumonia through regulating Keap1/Nrf2-mediated autophagic pathway and mitochondrial oxidative stress. Free Radic Biol Med. 2021;168:247-257 pubmed publisher
Gai C, Liu C, Wu X, Yu M, Zheng J, Zhang W, et al. MT1DP loaded by folate-modified liposomes sensitizes erastin-induced ferroptosis via regulating miR-365a-3p/NRF2 axis in non-small cell lung cancer cells. Cell Death Dis. 2020;11:751 pubmed publisher
Fischhuber K, Matzinger M, Heiss E. AMPK Enhances Transcription of Selected Nrf2 Target Genes via Negative Regulation of Bach1. Front Cell Dev Biol. 2020;8:628 pubmed publisher
Matzinger M, Fischhuber K, Pölöske D, Mechtler K, Heiss E. AMPK leads to phosphorylation of the transcription factor Nrf2, tuning transactivation of selected target genes. Redox Biol. 2019;29:101393 pubmed publisher
Tang X, Fu X, Liu Y, Yu D, Cai S, Yang C. Blockade of glutathione metabolism in IDH1-mutated glioma. Mol Cancer Ther. 2019;: pubmed publisher
Czaniecki C, Ryan T, Stykel M, Drolet J, Heide J, Hallam R, et al. Axonal pathology in hPSC-based models of Parkinson's disease results from loss of Nrf2 transcriptional activity at the Map1b gene locus. Proc Natl Acad Sci U S A. 2019;: pubmed publisher
Jena K, Kolapalli S, Mehto S, Nath P, Das B, Sahoo P, et al. TRIM16 controls assembly and degradation of protein aggregates by modulating the p62-NRF2 axis and autophagy. EMBO J. 2018;37: pubmed publisher
Hanmer K, Mavri Damelin D. Peroxidasin is a novel target of the redox-sensitive transcription factor Nrf2. Gene. 2018;674:104-114 pubmed publisher
Galiè M, Costanzo M, Nodari A, Boschi F, Calderan L, Mannucci S, et al. Mild ozonisation activates antioxidant cell response by the Keap1/Nrf2 dependent pathway. Free Radic Biol Med. 2018;124:114-121 pubmed publisher
Yang T, Sun Y, Mao L, Zhang M, Li Q, Zhang L, et al. Brain ischemic preconditioning protects against ischemic injury and preserves the blood-brain barrier via oxidative signaling and Nrf2 activation. Redox Biol. 2018;17:323-337 pubmed publisher
Khan N, Ahmad I, Haqqi T. Nrf2/ARE pathway attenuates oxidative and apoptotic response in human osteoarthritis chondrocytes by activating ERK1/2/ELK1-P70S6K-P90RSK signaling axis. Free Radic Biol Med. 2018;116:159-171 pubmed publisher
Milanese C, Tapias V, Gabriels S, Cerri S, Levandis G, Blandini F, et al. Mitochondrial Complex I Reversible S-Nitrosation Improves Bioenergetics and Is Protective in Parkinson's Disease. Antioxid Redox Signal. 2018;28:44-61 pubmed publisher
Ngo H, Kim D, Cha Y, Na H, Surh Y. Nrf2 Mutagenic Activation Drives Hepatocarcinogenesis. Cancer Res. 2017;77:4797-4808 pubmed publisher
Parvez S, Long M, Lin H, Zhao Y, Haegele J, Pham V, et al. T-REX on-demand redox targeting in live cells. Nat Protoc. 2016;11:2328-2356 pubmed publisher
Zanotto Filho A, Masamsetti V, Loranc E, Tonapi S, Gorthi A, Bernard X, et al. Alkylating Agent-Induced NRF2 Blocks Endoplasmic Reticulum Stress-Mediated Apoptosis via Control of Glutathione Pools and Protein Thiol Homeostasis. Mol Cancer Ther. 2016;15:3000-3014 pubmed
de Miranda Ramos V, Zanotto Filho A, de Bittencourt Pasquali M, Klafke K, Gasparotto J, Dunkley P, et al. NRF2 Mediates Neuroblastoma Proliferation and Resistance to Retinoic Acid Cytotoxicity in a Model of In Vitro Neuronal Differentiation. Mol Neurobiol. 2016;53:6124-6135 pubmed publisher
Kigoshi Y, Fukuda T, Endo T, Hayasaka N, Iemura S, Natsume T, et al. CACUL1/CAC1 Regulates the Antioxidant Response by Stabilizing Nrf2. Sci Rep. 2015;5:12857 pubmed publisher
Brzoska K, Stępkowski T, Kruszewski M. Basal PIR expression in HeLa cells is driven by NRF2 via evolutionary conserved antioxidant response element. Mol Cell Biochem. 2014;389:99-111 pubmed publisher
Anwar Mohamed A, Elshenawy O, Soshilov A, Denison M, Chris Le X, Klotz L, et al. Methylated pentavalent arsenic metabolites are bifunctional inducers, as they induce cytochrome P450 1A1 and NAD(P)H:quinone oxidoreductase through AhR- and Nrf2-dependent mechanisms. Free Radic Biol Med. 2014;67:171-87 pubmed publisher
Kanzaki H, Shinohara F, Kajiya M, Kodama T. The Keap1/Nrf2 protein axis plays a role in osteoclast differentiation by regulating intracellular reactive oxygen species signaling. J Biol Chem. 2013;288:23009-20 pubmed publisher
Furukawa M, Xiong Y. BTB protein Keap1 targets antioxidant transcription factor Nrf2 for ubiquitination by the Cullin 3-Roc1 ligase. Mol Cell Biol. 2005;25:162-71 pubmed
product information
Catalog Number :
21549
Product Name :
pcDNA3-EGFP-C4-Nrf2
article :
doi10.1128/MCB.25.1.162-171.2005
id2686
pubmed_id15601839
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size6112
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
NFE2L2
NRF2
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5BamHI
promoter
sequencing_primer_3SP6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHEBP1, IMDDHH, NRF2, Nrf-2
geneNFE2L2
id4780
genbank_ids
Q16236
mutation
namenuclear factor (erythroid-derived 2)-like 2
shRNA_sequence
size1825
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP
locationN terminal on backbone
tagMyc
resistance markers :
593
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA