This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
FLAG-Ago2
catalog :
21538
citations: 8
Reference
Sallis S, B xe9 rub xe9 Simard F, Grondin B, Leduc E, Azouz F, B xe9 langer C, et al. The CHARGE syndrome-associated protein FAM172A controls AGO2 nuclear import. Life Sci Alliance. 2023;6: pubmed publisher
Zhang Z, Yu T, Li H, Du L, Jin Z, Peng X, et al. Long Noncoding RNA AROD Inhibits Host Antiviral Innate Immunity via the miR-324-5p-CUEDC2 Axis. Microbiol Spectr. 2023;11:e0420622 pubmed publisher
Li M, Zhang S, Ma Y, Yang Y, An R. Role of hsa‑miR‑105 during the pathogenesis of paclitaxel resistance and its clinical implication in ovarian cancer. Oncol Rep. 2021;45: pubmed publisher
Ghosh S, Chakraborty J, Goswami A, Bhowmik S, Roy S, Ghosh A, et al. A novel microRNA boosts hyper-β-oxidation of fatty acids in liver by impeding CEP350-mediated sequestration of PPARα and thus restricts chronic hepatitis C. RNA Biol. 2020;17:1352-1363 pubmed publisher
Unal O, Akkoç Y, Kocak M, Nalbat E, Dogan Ekici A, Yagci Acar H, et al. Treatment of breast cancer with autophagy inhibitory microRNAs carried by AGO2-conjugated nanoparticles. J Nanobiotechnology. 2020;18:65 pubmed publisher
Seth P, Hsieh P, Jamal S, Wang L, Gygi S, Jain M, et al. Regulation of MicroRNA Machinery and Development by Interspecies S-Nitrosylation. Cell. 2019;176:1014-1025.e12 pubmed publisher
Katsushima K, Natsume A, Ohka F, Shinjo K, Hatanaka A, Ichimura N, et al. Targeting the Notch-regulated non-coding RNA TUG1 for glioma treatment. Nat Commun. 2016;7:13616 pubmed publisher
Lian S, Li S, Abadal G, Pauley B, Fritzler M, Chan E. The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing. RNA. 2009;15:804-13 pubmed publisher
product information
Catalog Number :
21538
Product Name :
FLAG-Ago2
article :
doi10.1261/rna.1229409
id2681
pubmed_id19324964
bacterial resistance :
Ampicillin
cloning :
backbonep3XFLAG-myc-CMV-24
backbone_mutation
backbone_origin
backbone_size4752
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The first 7aa of our Ago2 protein are different from NCBI website Ref seq. Addgene NGS results identified an R372M variant in Ago2.
origin :
37
pi :
alt_names
EIF2C2
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5CMV24sp
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10
geneAGO2
id27161
genbank_ids
NM_012154
mutationThe first 7aa of this Ago2 protein are different from the NCBI website Ref sequ
nameAgo2
shRNA_sequence
size2600
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag3XFLAG
plasmid copy :
Original Ago2 clone from Dr. Tom Hobman at Univ. of Calgary
resistance markers :
73
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA