This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
P-miR-145 (pTransluc-miR-145-1)
catalog :
21496
citations: 2
Reference
Panza A, Votino C, Gentile A, Valvano M, Colangelo T, Pancione M, et al. Peroxisome proliferator-activated receptor ?-mediated induction of microRNA-145 opposes tumor phenotype in colorectal cancer. Biochim Biophys Acta. 2014;1843:1225-36 pubmed publisher
Xu N, Papagiannakopoulos T, Pan G, Thomson J, Kosik K. MicroRNA-145 regulates OCT4, SOX2, and KLF4 and represses pluripotency in human embryonic stem cells. Cell. 2009;137:647-58 pubmed publisher
product information
Catalog Number :
21496
Product Name :
P-miR-145 (pTransluc-miR-145-1)
article :
doi10.1016/j.cell.2009.02.038
id2675
pubmed_id19409607
bacterial resistance :
Ampicillin
cloning :
backbonepTransluc (Panomics)
backbone_mutation
backbone_origin
backbone_size4800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Luciferase
growth notes :
Plasmid 21496: P-miR-145 (pTransluc-miR-145-1) has a mutation in the miR-145 sequence resulting in A to G mutation. The mutation is a SNP from the human ES cells H9.
growth temp :
DH5alpha
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Bgl II
cloning_site_5NheI
promoter
sequencing_primer_3
sequencing_primer_5ggtaccgagctcttacgcgt sequencing primer sequence
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIRN145, miR-145, miRNA145
geneMIR145
id406937
genbank_ids
mutation
name1.5kb 5' of miR-145
shRNA_sequence
size1500
species
9606
Homo sapiens
tags
resistance markers :
265
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA