This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMBP-HIS2-Vps20FL
catalog :
21490
citations: 4
Reference
Booth A, Marklew C, Ciani B, Beales P. The influence of phosphatidylserine localisation and lipid phase on membrane remodelling by the ESCRT-II/ESCRT-III complex. Faraday Discuss. 2021;232:188-202 pubmed publisher
Chiaruttini N, Redondo Morata L, Colom A, Humbert F, Lenz M, Scheuring S, et al. Relaxation of Loaded ESCRT-III Spiral Springs Drives Membrane Deformation. Cell. 2015;163:866-79 pubmed publisher
Bhutta M, Roy B, Gould G, McInerny C. A complex network of interactions between mitotic kinases, phosphatases and ESCRT proteins regulates septation and membrane trafficking in S. pombe. PLoS ONE. 2014;9:e111789 pubmed publisher
Wollert T, Wunder C, Lippincott Schwartz J, Hurley J. Membrane scission by the ESCRT-III complex. Nature. 2009;458:172-7 pubmed publisher
product information
Catalog Number :
21490
Product Name :
pMBP-HIS2-Vps20FL
article :
doi10.1038/nature07836
id2650
pubmed_id19234443
bacterial resistance :
Ampicillin
cloning :
backbonepHis2
backbone_mutation
backbone_origin
backbone_size5549
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
origin :
37
pi :
alt_names
CHM6
VPT20
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5N/A (RF cloning used)
promoter
sequencing_primer_3
sequencing_primer_5T7 promotor
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYMR077C, CHM6, VPL10, VPT20
geneVPS20
id855101
genbank_ids
mutationnone
nameVPS20
shRNA_sequence
size663
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on backbone
tag6x His
locationN terminal on backbone
tagMBP
resistance markers :
216
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA