This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHIV-Luciferase
catalog :
21375
citations: 21
Reference
Liang T, Xiao S, Wu Z, Lv X, Liu S, Hu M, et al. Phenothiazines Inhibit SARS-CoV-2 Entry through Targeting Spike Protein. Viruses. 2023;15: pubmed publisher
Alhilli M, Rhoades E, Chau D, Tewari S, Reich A, Myers A, et al. Unrestricted Ketogenic Diet Feeding Enhances Epithelial Ovarian Cancer Growth In Vivo. Nutrients. 2023;15: pubmed publisher
Moon J, Jung Y, Moon S, Hwang J, Kim S, Kim M, et al. Production and characterization of lentivirus vector-based SARS-CoV-2 pseudoviruses with dual reporters: Evaluation of anti-SARS-CoV-2 viral effect of Korean Red Ginseng. J Ginseng Res. 2023;47:123-132 pubmed publisher
Yu F, Pan T, Huang F, Ying R, Liu J, Fan H, et al. Glycopeptide Antibiotic Teicoplanin Inhibits Cell Entry of SARS-CoV-2 by Suppressing the Proteolytic Activity of Cathepsin L. Front Microbiol. 2022;13:884034 pubmed publisher
Varghese P, Kishore U, Rajkumari R. Human C1q Regulates Influenza A Virus Infection and Inflammatory Response via Its Globular Domain. Int J Mol Sci. 2022;23: pubmed publisher
Yuan Y, Zhang X, Chen R, Li Y, Wu B, Li R, et al. A bivalent nanoparticle vaccine exhibits potent cross-protection against the variants of SARS-CoV-2. Cell Rep. 2021;:110256 pubmed publisher
Varghese P, Mukherjee S, Al Mohanna F, Saleh S, Almajhdi F, Beirag N, et al. Human Properdin Released By Infiltrating Neutrophils Can Modulate Influenza A Virus Infection. Front Immunol. 2021;12:747654 pubmed publisher
Pan T, Chen R, He X, Yuan Y, Deng X, Li R, et al. Infection of wild-type mice by SARS-CoV-2 B.1.351 variant indicates a possible novel cross-species transmission route. Signal Transduct Target Ther. 2021;6:420 pubmed publisher
Varghese P, Murugaiah V, Beirag N, Temperton N, Khan H, Alrokayan S, et al. C4b Binding Protein Acts as an Innate Immune Effector Against Influenza A Virus. Front Immunol. 2020;11:585361 pubmed publisher
Ma X, Zou F, Yu F, Li R, Yuan Y, Zhang Y, et al. Nanoparticle Vaccines Based on the Receptor Binding Domain (RBD) and Heptad Repeat (HR) of SARS-CoV-2 Elicit Robust Protective Immune Responses. Immunity. 2020;53:1315-1330.e9 pubmed publisher
Chuang T, Rehan A, Khorram O. Functional role of the long noncoding RNA X-inactive specific transcript in leiomyoma pathogenesis. Fertil Steril. 2021;115:238-247 pubmed publisher
Yu L, Wang L, Kim J, Mao C, Shapiro D. Src couples estrogen receptor to the anticipatory unfolded protein response and regulates cancer cell fate under stress. Biochim Biophys Acta Mol Cell Res. 2020;:118765 pubmed publisher
Chambers L, Esakov E, Braley C, Alhilli M, Michener C, Reizes O. Use of Transabdominal Ultrasound for the detection of intra-peritoneal tumor engraftment and growth in mouse xenografts of epithelial ovarian cancer. PLoS ONE. 2020;15:e0228511 pubmed publisher
Murugaiah V, Varghese P, Saleh S, Tsolaki A, Alrokayan S, Khan H, et al. Complement-Independent Modulation of Influenza A Virus Infection by Factor H. Front Immunol. 2020;11:355 pubmed publisher
Lillo Osuna M, García López J, El Ayachi I, Fatima I, Khalid A, Kumpati J, et al. Activation of Estrogen Receptor Alpha by Decitabine Inhibits Osteosarcoma Growth and Metastasis. Cancer Res. 2019;79:1054-1068 pubmed publisher
Scarfe L, Taylor A, Sharkey J, Harwood R, Barrow M, Comenge J, et al. Non-invasive imaging reveals conditions that impact distribution and persistence of cells after in vivo administration. Stem Cell Res Ther. 2018;9:332 pubmed publisher
Yu L, Wang L, Mao C, Duraki D, Kim J, Huang R, et al. Estrogen-independent Myc overexpression confers endocrine therapy resistance on breast cancer cells expressing ERαY537S and ERαD538G mutations. Cancer Lett. 2019;442:373-382 pubmed publisher
Al Ahdal M, Murugaiah V, Varghese P, Abozaid S, Saba I, Al Qahtani A, et al. Entry Inhibition and Modulation of Pro-Inflammatory Immune Response Against Influenza A Virus by a Recombinant Truncated Surfactant Protein D. Front Immunol. 2018;9:1586 pubmed publisher
Comenge J, Sharkey J, Fragueiro O, Wilm B, Brust M, Murray P, et al. Multimodal cell tracking from systemic administration to tumour growth by combining gold nanorods and reporter genes. elife. 2018;7: pubmed publisher
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Ampuja M, Alarmo E, Owens P, Havunen R, Gorska A, Moses H, et al. The impact of bone morphogenetic protein 4 (BMP4) on breast cancer metastasis in a mouse xenograft model. Cancer Lett. 2016;375:238-244 pubmed publisher
product information
Catalog Number :
21375
Product Name :
pHIV-Luciferase
article :
doi
id2660
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepSICO
backbone_mutation
backbone_origin
backbone_size8713
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
The HIV-Luciferase plasmid encodes a self-inactivating lentiviral construct that was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and Luciferase. A cDNA can be cloned into the MCS (NotI, EcoRI, SmaI, and BamHI sites) to enable bicistronic expression with luciferase. This construct has not been published. NOTE: Addgene's stock is missing bp 6102-6190 compared to the original depositor's sequence. This deletion may affect some restriction sites, but not plasmid function.
growth strain :
self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase
growth temp :
Growth in Stbl3 is preferred
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_35'-AGGAACTGCTTCCTTCACGA-3'
sequencing_primer_55'-TGGAATTTGCCCTTTTTGAG-3'
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
name
shRNA_sequence
size
species
tags
plasmid copy :
pSICO was obtained from Tyler Jacks laboratory.
resistance markers :
573
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA