This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLKO human shRNA 2 DEPTOR
catalog :
21336
citations: 1
Reference
Peterson T, Laplante M, Thoreen C, Sancak Y, Kang S, Kuehl W, et al. DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for their survival. Cell. 2009;137:873-86 pubmed publisher
product information
Catalog Number :
21336
Product Name :
pLKO human shRNA 2 DEPTOR
article :
doi10.1016/j.cell.2009.03.046
id2654
pubmed_id19446321
bacterial resistance :
Ampicillin
cloning :
backbonepLKO.1
backbone_mutation
backbone_originAvailable from Addgene
backbone_size7032
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
RNAi
growth notes :
5'-CCGGGCAAGGAAGACATTCACGATTCTCGAGAATCG
TGAATGTCTTCCTTGCTTTTTG
human DEPTOR shRNA 2 TRC candidate; NM_022783.1-1101s1c1 shRNA oligo sequence -
growth temp :
XL10 Gold Ultracompetent Cells from Stratagene or nStb13. Grow in SOC media at 30oC. Can be tough to grow.
origin :
30
pi :
alt_names
DEPDC6
DEPTOR
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5AgeI
promoter
sequencing_primer_3
sequencing_primer_5LKO.1 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDEP.6, DEPDC6
geneDEPTOR
id64798
genbank_ids
mutation
nameDEPTOR shRNA 2
shRNA_sequence
size60
species
9606
Homo sapiens
tags
resistance markers :
432
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA