This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRK5 FLAG human DEPTOR
catalog :
21334
citations: 13
Reference
Ouyang Z, Kang D, Li K, Liang G, Liu Z, Mai Q, et al. DEPTOR exacerbates bone-fat imbalance in osteoporosis by transcriptionally modulating BMSC differentiation. Biomed Pharmacother. 2022;151:113164 pubmed publisher
Wälchli M, Berneiser K, Mangia F, Imseng S, Craigie L, Stuttfeld E, et al. Regulation of human mTOR complexes by DEPTOR. elife. 2021;10: pubmed publisher
Kim S, Lee S, Kim Y, Park J, Woo D, Kim D, et al. Tanc2-mediated mTOR inhibition balances mTORC1/2 signaling in the developing mouse brain and human neurons. Nat Commun. 2021;12:2695 pubmed publisher
Chawsheen M, Dash P. mTOR modulates resistance to gemcitabine in lung cancer in an MTORC2 dependent mechanism. Cell Signal. 2021;81:109934 pubmed publisher
Li K, Yang P, Zhang Y, Zhang Y, Cao H, Liu P, et al. DEPTOR Prevents Osteoarthritis Development Via Interplay With TRC8 to Reduce Endoplasmic Reticulum Stress in Chondrocytes. J Bone Miner Res. 2020;: pubmed publisher
Wang C, Dai H, Xiong Z, Song Q, Zou Z, Li M, et al. Loss of DEPTOR in renal tubules protects against cisplatin-induced acute kidney injury. Cell Death Dis. 2018;9:441 pubmed publisher
Ding Y, Shan L, Nai W, Lin X, Zhou L, Dong X, et al. DEPTOR Deficiency-Mediated mTORc1 Hyperactivation in Vascular Endothelial Cells Promotes Angiogenesis. Cell Physiol Biochem. 2018;46:520-531 pubmed publisher
Brown M, Gromeier M. MNK Controls mTORC1:Substrate Association through Regulation of TELO2 Binding with mTORC1. Cell Rep. 2017;18:1444-1457 pubmed publisher
Catena V, Bruno T, De Nicola F, Goeman F, Pallocca M, Iezzi S, et al. Deptor transcriptionally regulates endoplasmic reticulum homeostasis in multiple myeloma cells. Oncotarget. 2016;7:70546-70558 pubmed publisher
Carbonneau M, M Gagné L, Lalonde M, Germain M, Motorina A, Guiot M, et al. The oncometabolite 2-hydroxyglutarate activates the mTOR signalling pathway. Nat Commun. 2016;7:12700 pubmed publisher
Srinivas K, Viji R, Dan V, Sajitha I, Prakash R, Rahul P, et al. DEPTOR promotes survival of cervical squamous cell carcinoma cells and its silencing induces apoptosis through downregulating PI3K/AKT and by up-regulating p38 MAP kinase. Oncotarget. 2016;7:24154-71 pubmed publisher
González Terán B, López J, Rodríguez E, Leiva L, Martínez Martínez S, Bernal J, et al. p38γ and δ promote heart hypertrophy by targeting the mTOR-inhibitory protein DEPTOR for degradation. Nat Commun. 2016;7:10477 pubmed publisher
Peterson T, Laplante M, Thoreen C, Sancak Y, Kang S, Kuehl W, et al. DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for their survival. Cell. 2009;137:873-86 pubmed publisher
product information
Catalog Number :
21334
Product Name :
pRK5 FLAG human DEPTOR
article :
doi10.1016/j.cell.2009.03.046
id2654
pubmed_id19446321
bacterial resistance :
Ampicillin
cloning :
backbonepRK5-FLAG
backbone_mutation
backbone_origin
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Wild type full length FLAG tagged DEPTOR cDNA in pRK5
origin :
37
pi :
alt_names
DEPDC6
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5SalI
promoter
sequencing_primer_3SV40pA-R
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDEP.6, DEPDC6
geneDEPTOR
id64798
genbank_ids
NM_022783
mutationN204S and S389N mutations (numbering according to associated publication)
nameDEPTOR
shRNA_sequence
size1300
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFLAG
resistance markers :
432
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA