This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PL-SIN-EF1 -EGFP
catalog :
21320
citations: 14
Reference
Hidalgo L, Somovilla Crespo B, Garcia Rodriguez P, Morales Molina Á, Rodríguez Milla M, Garcia Castro J. Switchable CAR T cell strategy against osteosarcoma. Cancer Immunol Immunother. 2023;72:2623-2633 pubmed publisher
Briukhovetska D, Suarez Gosalvez J, Voigt C, Markota A, Giannou A, Sch xfc bel M, et al. T cell-derived interleukin-22 drives the expression of CD155 by cancer cells to suppress NK cell function and promote metastasis. Immunity. 2023;56:143-161.e11 pubmed publisher
Nakajima K, Shen Z, Miura M, Nakabayashi H, Kawahara M. Sequential control of myeloid cell proliferation and differentiation by cytokine receptor-based chimeric antigen receptors. PLoS ONE. 2022;17:e0279409 pubmed publisher
Mok R, Zhang W, Sheikh T, Pradeepan K, Fernandes I, DeJong L, et al. Wide spectrum of neuronal and network phenotypes in human stem cell-derived excitatory neurons with Rett syndrome-associated MECP2 mutations. Transl Psychiatry. 2022;12:450 pubmed publisher
Namous H, Braz C, Wang Y, Khatib H. The Activation of Protamine 1 Using Epigenome Editing Decreases the Proliferation of Tumorigenic Cells. Front Genome Ed. 2022;4:844904 pubmed publisher
Ramírez Torres A, Gil J, Contreras S, Ramírez G, Valencia González H, Salazar Bustamante E, et al. Quantitative Proteomic Analysis of Cervical Cancer Tissues Identifies Proteins Associated With Cancer Progression. Cancer Genomics Proteomics. 2022;19:241-258 pubmed publisher
Díaz Tejeda Y, Guido Jimenez M, López Carbajal H, Amador Molina A, Méndez Martínez R, Gariglio Vidal P, et al. Nanog, in Cooperation with AP1, Increases the Expression of E6/E7 Oncogenes from HPV Types 16/18. Viruses. 2021;13: pubmed publisher
Katz D, Huynh N, Savadipour A, Palte I, Guilak F. An immortalized human adipose-derived stem cell line with highly enhanced chondrogenic properties. Biochem Biophys Res Commun. 2020;530:252-258 pubmed publisher
Ruiz G, Valencia González H, Perez Montiel D, Muñoz F, Ocadiz Delgado R, Fernandez Retana J, et al. Genes Involved in the Transcriptional Regulation of Pluripotency Are Expressed in Malignant Tumors of the Uterine Cervix and Can Induce Tumorigenic Capacity in a Nontumorigenic Cell Line. Stem Cells Int. 2019;2019:7683817 pubmed publisher
Peskova L, Cerná K, Oppelt J, Mraz M, Bárta T. Oct4-mediated reprogramming induces embryonic-like microRNA expression signatures in human fibroblasts. Sci Rep. 2019;9:15759 pubmed publisher
Nakabayashi H, Aoyama S, Kawahara M, Nagamune T. Differentiation signalobody: Demonstration of antigen-dependent osteoclast differentiation from a progenitor cell line. J Biosci Bioeng. 2016;122:357-63 pubmed publisher
Hilton I, D Ippolito A, Vockley C, Thakore P, Crawford G, Reddy T, et al. Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 2015;33:510-7 pubmed publisher
Brunger J, Huynh N, Guenther C, Perez Pinera P, Moutos F, Sanchez Adams J, et al. Scaffold-mediated lentiviral transduction for functional tissue engineering of cartilage. Proc Natl Acad Sci U S A. 2014;111:E798-806 pubmed publisher
Hotta A, Cheung A, Farra N, Vijayaragavan K, Séguin C, Draper J, et al. Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency. Nat Methods. 2009;6:370-6 pubmed publisher
product information
Catalog Number :
21320
Product Name :
PL-SIN-EF1 -EGFP
article :
doi10.1038/nmeth.1325
id2648
pubmed_id19404254
bacterial resistance :
Ampicillin
cloning :
backbonePL-SIN lentiviral vector
backbone_mutation
backbone_originBuzina et al., PLoS Genetics, 2008
backbone_size6881
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
EGFP driven by ubiquitous EF1 promoter. PL-SIN lentiviral vector (Buzina et al., PLoS Genetics, 2008) requires Tat cDNA for lentivirus production, as well as other packaging cDNAs (Gag/pol, Rev, and VSV-G).
growth strain :
Control Lentiviral vector with strong EF1a promoter, expresses EGFP
origin :
37
pi :
alt_names
EGFP
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterEF1
sequencing_primer_3n/a
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameEnhanced Green Fluorescence Protein
shRNA_sequence
size720
species
tags
resistance markers :
134
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA