This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLew100::NLS-ISceI-HA
catalog :
21299
citations: 1
Reference
Boothroyd C, Dreesen O, Leonova T, Ly K, Figueiredo L, Cross G, et al. A yeast-endonuclease-generated DNA break induces antigenic switching in Trypanosoma brucei. Nature. 2009;459:278-81 pubmed publisher
product information
Catalog Number :
21299
Product Name :
pLew100::NLS-ISceI-HA
article :
doi10.1038/nature07982
id2642
pubmed_id19369939
bacterial resistance :
Ampicillin
cloning :
backbonepLew100
backbone_mutation
backbone_origin
backbone_size4300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
There are a few discrepancies between Addgene's sequence and the provided sequence from the depositing lab. These discrepancies do not affect plasmid function.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5HindIII
promoter
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesQ0160
geneSCEI
id854590
genbank_ids
FJ797515
mutation
nameI-SceI
shRNA_sequence
size2000
species
tags
locationN terminal on insert
tagNLS
locationC terminal on insert
tagHA
resistance markers :
379
tags :
Unknown
terms :
Zeocin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA