This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
zWnt5b
catalog :
21282
citations: 2
| Reference |
|---|
Stoick Cooper C, Weidinger G, Riehle K, Hubbert C, Major M, Fausto N, et al. Distinct Wnt signaling pathways have opposing roles in appendage regeneration. Development. 2007;134:479-89 pubmed
|
product information
Catalog Number :
21282
Product Name :
zWnt5b
article :
| doi | 10.1242/dev.001123 |
| id | 2635 |
| pubmed_id | 17185322 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pBluescript |
| backbone_mutation | |
| backbone_origin | Stratagene |
| backbone_size | 3000 |
| promoter | |
| sequencing_primer_3 | |
| sequencing_primer_5 | |
| vector_types |
growth notes :
Zebrafish wnt5 (pipetail) cDNA for antisense probe. Full-length ORF plus some (exact sequence not known, likely genbank NM_130937) 5' and 3' UTRs, 1.75kb total insert. Cloned into EcoRI site of pBluescript (there is also an internal EcoRI!). From Phil Inghams' lab, cloned by P. Blader. Used to be called wnt5 and consider by many people to be wnt5a. however, after Gilbert cloned the real wnt5a, it is now known as wnt5b. For antisense zWnt5b: cut w/ XbaI, transcribe w/ T3.
origin :
37
pi :
|
plasmid copy :
From Phil Inghams' lab, cloned by P. Blader.
resistance markers :
| 348 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments