This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
aMHC-mCherry-Rex-Blasticidin
catalog :
21228
citations: 5
Reference
Lee J, An J, Taniguchi J, Kashiwazaki G, Pandian G, Parveen N, et al. Targeted epigenetic modulation using a DNA-based histone deacetylase inhibitor enhances cardiomyogenesis in mouse embryonic stem cells. J Cell Physiol. 2020;: pubmed publisher
Lee J, An J, Kang T, De D, Kim K. Discovery of Natural Compounds Promoting Cardiomyocyte Differentiation. Stem Cells Dev. 2019;28:13-27 pubmed publisher
Leach L, Buchholz D, Nadar V, Lowenstein S, Clegg D. Canonical/β-catenin Wnt pathway activation improves retinal pigmented epithelium derivation from human embryonic stem cells. Invest Ophthalmol Vis Sci. 2015;56:1002-13 pubmed publisher
Maass K, Shekhar A, Lu J, Kang G, See F, Kim E, et al. Isolation and characterization of embryonic stem cell-derived cardiac Purkinje cells. Stem Cells. 2015;33:1102-12 pubmed publisher
Kita Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot J, et al. Lentiviral vectors and protocols for creation of stable hESC lines for fluorescent tracking and drug resistance selection of cardiomyocytes. PLoS ONE. 2009;4:e5046 pubmed publisher
product information
Catalog Number :
21228
Product Name :
aMHC-mCherry-Rex-Blasticidin
article :
doi10.1371/journal.pone.0005046
id2562
pubmed_id19352491
bacterial resistance :
Ampicillin
cloning :
backboneSMPU
backbone_mutation
backbone_origin
backbone_size7338
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth notes :
There is an extra C in the promoter sequence when compared to the available reference sequence in NCBI. The mismatch is due to older sequencing artifact of an erroneous insertion of a C adjacent to GGG . The construct should be correct.
origin :
37
pi :
alt_names
aMHC promoter
cloning
clone_methodRestriction Enzyme
cloning_site_3AgeI
cloning_site_5BamHI
promoter
sequencing_primer_3
sequencing_primer_5CTT GAA GCG CAT GAA CTC - 3' Sequencing only
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namealpha myosin heavy chain promoter
shRNA_sequence
size5470
species
10090
Mus musculus
tags
locationC terminal on backbone
tagmCherry
resistance markers :
330
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA