This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PGK-H2BeGFP
catalog :
21210
citations: 8
Reference
Pond K, Morris J, Alkhimenok O, Varghese R, Cabel C, Ellis N, et al. Live-cell imaging in human colonic monolayers reveals ERK waves limit the stem cell compartment to maintain epithelial homeostasis. elife. 2022;11: pubmed publisher
Yedier Bayram O, Gokbayrak B, Kayabolen A, Aksu A, Cavga A, Cing xf6 z A, et al. EPIKOL, a chromatin-focused CRISPR/Cas9-based screening platform, to identify cancer-specific epigenetic vulnerabilities. Cell Death Dis. 2022;13:710 pubmed publisher
Chinn H, Gardell J, Matsumoto L, Labadie K, Mihailovic T, Lieberman N, et al. Hypoxia-inducible lentiviral gene expression in engineered human macrophages. J Immunother Cancer. 2022;10: pubmed publisher
Davey R, Digman M, Gratton E, Moens P. Quantitative image mean squared displacement (iMSD) analysis of the dynamics of profilin 1 at the membrane of live cells. Methods. 2018;140-141:119-125 pubmed publisher
Kilinc D, Schwab J, Rampini S, Ikpekha O, Thampi A, Blasiak A, et al. A microfluidic dual gradient generator for conducting cell-based drug combination assays. Integr Biol (Camb). 2016;8:39-49 pubmed publisher
Venugopalan G, Camarillo D, Webster K, Reber C, Sethian J, Weaver V, et al. Multicellular architecture of malignant breast epithelia influences mechanics. PLoS ONE. 2014;9:e101955 pubmed publisher
Kreitzer F, Salomonis N, Sheehan A, Huang M, Park J, Spindler M, et al. A robust method to derive functional neural crest cells from human pluripotent stem cells. Am J Stem Cells. 2013;2:119-31 pubmed
Kita Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot J, et al. Lentiviral vectors and protocols for creation of stable hESC lines for fluorescent tracking and drug resistance selection of cardiomyocytes. PLoS ONE. 2009;4:e5046 pubmed publisher
product information
Catalog Number :
21210
Product Name :
PGK-H2BeGFP
article :
doi10.1371/journal.pone.0005046
id2562
pubmed_id19352491
bacterial resistance :
Ampicillin
cloning :
backboneSIN18
backbone_mutation
backbone_origin
backbone_size7226
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth notes :
There are several mismatches between depositor's and Addgene sequence. These mutations do not affect eGFP expression.
origin :
37
pi :
alt_names
PGK promoter
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5XhoI
promoter
sequencing_primer_3CTTGATGCCGTTCTTCTGCTT
sequencing_primer_5ACC CTC GCA GAC GGA CAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHEL-S-68p, MIG10, PGKA
genePGK1
id5230
genbank_ids
mutation
namePhosphoglycerate kinase promoter
shRNA_sequence
size541
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagH2B-eGFP
resistance markers :
330
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA