This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
L1E-1
catalog :
21193
citations: 3
Reference
Patel H, Mishra R, Wier A, Mokhtarpour N, Merino E, Garrett J. RIDR-PI-103, ROS-activated prodrug PI3K inhibitor inhibits cell growth and impairs the PI3K/Akt pathway in BRAF and MEK inhibitor-resistant BRAF-mutant melanoma cells. Anticancer Drugs. 2023;34:519-531 pubmed publisher
Ravi D, Bhalla S, Gartenhaus R, Crombie J, Kandela I, Sharma J, et al. The novel organic arsenical darinaparsin induces MAPK-mediated and SHP1-dependent cell death in T-cell lymphoma and Hodgkin lymphoma cells and human xenograft models. Clin Cancer Res. 2014;20:6023-33 pubmed publisher
Scholl F, Dumesic P, Khavari P. Mek1 alters epidermal growth and differentiation. Cancer Res. 2004;64:6035-40 pubmed
product information
Catalog Number :
21193
Product Name :
L1E-1
article :
doi10.1158/0008-5472.CAN-04-0017
id2632
pubmed_id15342384
bacterial resistance :
Ampicillin
cloning :
backboneLZRS-RfA
backbone_mutation
backbone_origin
backbone_size11100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Retroviral
growth notes :
Constitutive active protein has the following aa sequence deleted - ALQKKLEELELDEQQRKRLE, residues 31 to 51.
growth temp :
STBL2
origin :
37
pi :
alt_names
MAP2K1
ER-{alpha}
Esr1
cloning
clone_methodGateway Cloning
cloning_site_3attR
cloning_site_5attR
promoter
sequencing_primer_3ATCGTCGACCACTGTGCTGG
sequencing_primer_5TGGATACACGCCGCCCACGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesER, ER-alpha, ERa, ERalpha, ESR, Estr, Estra, Nr3a1
geneEsr1
id13982
aliasesCFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1
geneMAP2K1
id5604
genbank_ids
mutationMek1 R4F fused to ER*, constitutive active
nameMek1
shRNA_sequence
size2200
species
9606
Homo sapiens
10090
Mus musculus
tags
resistance markers :
249
tags :
Low Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA