pOTF3-AK (clone3) | Addgene 21165 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pOTF3-AK (clone3)

catalog :

21165

citations: 2

Reference
Bhadriraju K, Halter M, Amelot J, Bajcsy P, Chalfoun J, Vandecreme A, et al. Large-scale time-lapse microscopy of Oct4 expression in human embryonic stem cell colonies. Stem Cell Res. 2016;17:122-9 pubmed publisher
Zwaka T, Thomson J. Homologous recombination in human embryonic stem cells. Nat Biotechnol. 2003;21:319-21 pubmed

product information

Catalog Number :

21165

Product Name :

pOTF3-AK (clone3)

article :

doi	10.1038/nbt788
id	2598
pubmed_id	12577066

bacterial resistance :

Ampicillin

cloning :

backbone

pBluescript SK (-)

backbone_mutation

backbone_origin

Stratagene

backbone_size

2900

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Cre/Lox

Other

homologous recombination

growth notes :

The gene-targeting vector was constructed by insertion of an IRES-EGFP, and IRES-Neo, and an SV40 polyadenylation sequenc (aprox 3.2kb) into the 3' untranslated region of the fifth exon of the human POU5F1 gene

origin :

pi :

alt_names

Oct4

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	SalI
promoter
sequencing_primer_3	pLac
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
gene	POU5F1
id	5460

genbank_ids

AC006047

mutation

NeoR, EGFP, and SV40 polyA are between the two genomic sequences (one section is 1604 bp and one is 9022).

name

POUF5F1 (genomic DNA)

shRNA_sequence

size

13500

species

9606

Homo sapiens