This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSIN4-EF2-N2L
catalog :
21163
citations: 5
Reference
Rodriguez Gutierrez D, Eid W, Biason Lauber A. A Human Gonadal Cell Model From Induced Pluripotent Stem Cells. Front Genet. 2018;9:498 pubmed publisher
Mathieu J, Zhou W, Xing Y, Sperber H, Ferreccio A, Agoston Z, et al. Hypoxia-inducible factors have distinct and stage-specific roles during reprogramming of human cells to pluripotency. Cell Stem Cell. 2014;14:592-605 pubmed publisher
Onder T, Kara N, Cherry A, Sinha A, Zhu N, Bernt K, et al. Chromatin-modifying enzymes as modulators of reprogramming. Nature. 2012;483:598-602 pubmed publisher
Nethercott H, Brick D, Schwartz P. Derivation of induced pluripotent stem cells by lentiviral transduction. Methods Mol Biol. 2011;767:67-85 pubmed publisher
Yu J, Hu K, Smuga Otto K, Tian S, Stewart R, Slukvin I, et al. Human induced pluripotent stem cells free of vector and transgene sequences. Science. 2009;324:797-801 pubmed publisher
product information
Catalog Number :
21163
Product Name :
pSIN4-EF2-N2L
article :
doi10.1126/science.1172482
id2535
pubmed_id19325077
bacterial resistance :
Ampicillin
cloning :
backbonepSIN4-EF2-IRES-Pur
backbone_mutation
backbone_origin
backbone_size6300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
Other
reprogramming
growth notes :
After lentivirus infection, Nanog and Lin28 are coexpressed by IRES2 intron. Infection with this lentivirus, when used with pSIN4-EF2-O2S and pSIN4-CMV-K2M have a significantly greater reprogramming efficiency than our original 4 lentiviruses.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5SpeI
promoter
sequencing_primer_3
sequencing_primer_5EF1a_for
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneNANOG
id79923
aliasesCSDD1, LIN-28, LIN28, ZCCHC1, lin-28A
geneLIN28A
id79727
genbank_ids
NM_024865
NM_024674
mutation
nameNanog, Lin28
shRNA_sequence
size1548
species
9606
Homo sapiens
tags
resistance markers :
523
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA