This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSIN4-EF2-O2S
catalog :
21162
citations: 13
Reference
Uğurlu Çimen D, Odluyurt D, Sevinç K, Özkan Küçük N, Özçimen B, Demirtaş D, et al. AF10 (MLLT10) prevents somatic cell reprogramming through regulation of DOT1L-mediated H3K79 methylation. Epigenetics Chromatin. 2021;14:32 pubmed publisher
Güney Esken G, Erol Ö, Pervin B, Gürhan Sevinç G, Onder T, Bilgiç E, et al. Development, characterization, and hematopoietic differentiation of Griscelli syndrome type 2 induced pluripotent stem cells. Stem Cell Res Ther. 2021;12:287 pubmed publisher
Nakanishi M, Mitchell R, Benoit Y, Orlando L, Reid J, Shimada K, et al. Human Pluripotency Is Initiated and Preserved by a Unique Subset of Founder Cells. Cell. 2019;177:910-924.e22 pubmed publisher
Whitworth D, Limnios I, Gauthier M, Weeratunga P, Ovchinnikov D, Baillie G, et al. Platypus Induced Pluripotent Stem Cells: the Unique Pluripotency Signature of a Monotreme. Stem Cells Dev. 2018;: pubmed publisher
Rodriguez Gutierrez D, Eid W, Biason Lauber A. A Human Gonadal Cell Model From Induced Pluripotent Stem Cells. Front Genet. 2018;9:498 pubmed publisher
Lee J, Salci K, Reid J, Orlando L, Tanasijevic B, Shapovalova Z, et al. Brief Report: Human Acute Myeloid Leukemia Reprogramming to Pluripotency Is a Rare Event and Selects for Patient Hematopoietic Cells Devoid of Leukemic Mutations. Stem Cells. 2017;35:2095-2102 pubmed publisher
Nayler S, Kozlov S, Lavin M, Wolvetang E. Lentiviral Reprogramming of A-T Patient Fibroblasts to Induced Pluripotent Stem Cells. Methods Mol Biol. 2017;1599:401-418 pubmed publisher
Cheow L, Courtois E, Tan Y, Viswanathan R, Xing Q, Tan R, et al. Single-cell multimodal profiling reveals cellular epigenetic heterogeneity. Nat Methods. 2016;13:833-6 pubmed publisher
Mathieu J, Zhou W, Xing Y, Sperber H, Ferreccio A, Agoston Z, et al. Hypoxia-inducible factors have distinct and stage-specific roles during reprogramming of human cells to pluripotency. Cell Stem Cell. 2014;14:592-605 pubmed publisher
Whitworth D, Ovchinnikov D, Sun J, Fortuna P, Wolvetang E. Generation and characterization of leukemia inhibitory factor-dependent equine induced pluripotent stem cells from adult dermal fibroblasts. Stem Cells Dev. 2014;23:1515-23 pubmed publisher
Onder T, Kara N, Cherry A, Sinha A, Zhu N, Bernt K, et al. Chromatin-modifying enzymes as modulators of reprogramming. Nature. 2012;483:598-602 pubmed publisher
Nethercott H, Brick D, Schwartz P. Derivation of induced pluripotent stem cells by lentiviral transduction. Methods Mol Biol. 2011;767:67-85 pubmed publisher
Yu J, Hu K, Smuga Otto K, Tian S, Stewart R, Slukvin I, et al. Human induced pluripotent stem cells free of vector and transgene sequences. Science. 2009;324:797-801 pubmed publisher
product information
Catalog Number :
21162
Product Name :
pSIN4-EF2-O2S
article :
doi10.1126/science.1172482
id2535
pubmed_id19325077
bacterial resistance :
Ampicillin
cloning :
backbonepSIN4-EF2-IRES-Pur
backbone_mutation
backbone_origin
backbone_size6300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
Other
reprogramming
growth notes :
After lentivirus infection, Oct4 and Sox2 are coexpressed by EF2 promoter and IRES intron. Infection with this lentivirus, when used with pSIN4-EF2-N2L and pSIN4-CMV-K2M have a significantly greater reprogramming efficiency than our original 4 lentiviruses.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5SpeI
promoter
sequencing_primer_3
sequencing_primer_5EF1a_for
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesANOP3, MCOPS3
geneSOX2
id6657
aliasesOCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
genePOU5F1
id5460
genbank_ids
NM_002701
NM_003106
mutation
nameOct4, Sox2
shRNA_sequence
size2037
species
9606
Homo sapiens
tags
resistance markers :
523
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA