This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-HA-Sumo1
catalog :
21154
citations: 13
Reference
Rosemarie Q, Kirschstein E, Sugden B. How Epstein-Barr Virus Induces the Reorganization of Cellular Chromatin. MBio. 2023;:e0268622 pubmed publisher
Rauth S, Karmakar S, Shah A, Seshacharyulu P, Nimmakayala R, Ganguly K, et al. SUMO Modification of PAF1/PD2 Enables PML Interaction and Promotes Radiation Resistance in Pancreatic Ductal Adenocarcinoma. Mol Cell Biol. 2021;41:e0013521 pubmed publisher
Sá Pessoa J, Przybyszewska K, Vasconcelos F, Dumigan A, Frank C, Hobley L, et al. Klebsiella pneumoniae Reduces SUMOylation To Limit Host Defense Responses. MBio. 2020;11: pubmed publisher
Singh A, Khare P, Obaid A, Conlon K, Basrur V, Depinho R, et al. SUMOylation of ROR-γt inhibits IL-17 expression and inflammation via HDAC2. Nat Commun. 2018;9:4515 pubmed publisher
Appikonda S, Thakkar K, Shah P, Dent S, Andersen J, Barton M. Cross-talk between chromatin acetylation and SUMOylation of tripartite motif-containing protein 24 (TRIM24) impacts cell adhesion. J Biol Chem. 2018;293:7476-7485 pubmed publisher
Kuo C, Pai P, Ho T, Lin Y, Chen R, Tsai F, et al. Inhibition of HSF2 SUMOylation via MEL18 upregulates IGF-IIR and leads to hypertension-induced cardiac hypertrophy. Int J Cardiol. 2018;257:283-290 pubmed publisher
Yue X, Zhang C, Zhao Y, Liu J, Lin A, Tan V, et al. Gain-of-function mutant p53 activates small GTPase Rac1 through SUMOylation to promote tumor progression. Genes Dev. 2017;31:1641-1654 pubmed publisher
Yuan D, Meng Z, Xu K, Li Q, Chen C, Li K, et al. Betulinic acid increases radiosensitization of oral squamous cell carcinoma through inducing Sp1 sumoylation and PTEN expression. Oncol Rep. 2017;38:2360-2368 pubmed publisher
Liu Y, Zheng Z, Shu B, Meng J, Zhang Y, Zheng C, et al. SUMO Modification Stabilizes Enterovirus 71 Polymerase 3D To Facilitate Viral Replication. J Virol. 2016;90:10472-10485 pubmed publisher
Djavadian R, Chiu Y, Johannsen E. An Epstein-Barr Virus-Encoded Protein Complex Requires an Origin of Lytic Replication In Cis to Mediate Late Gene Transcription. PLoS Pathog. 2016;12:e1005718 pubmed publisher
Roy R, Durie D, Li H, Liu B, Skehel J, Mauri F, et al. hnRNPA1 couples nuclear export and translation of specific mRNAs downstream of FGF-2/S6K2 signalling. Nucleic Acids Res. 2014;42:12483-97 pubmed publisher
Dunphy P, Luo T, McBride J. Ehrlichia chaffeensis exploits host SUMOylation pathways to mediate effector-host interactions and promote intracellular survival. Infect Immun. 2014;82:4154-68 pubmed publisher
Terui Y, Saad N, Jia S, McKeon F, Yuan J. Dual role of sumoylation in the nuclear localization and transcriptional activation of NFAT1. J Biol Chem. 2004;279:28257-65 pubmed
product information
Catalog Number :
21154
Product Name :
pcDNA3-HA-Sumo1
article :
doi10.1074/jbc.M403153200
id2589
pubmed_id15117942
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1 HA
backbone_mutationBGH polyA terminator removed during cloning of Sumo1
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
This plasmid does not contain the BGH polyA terminator present in the standard pcDNA3 backbone. It is not known whether the removal of the terminator affects expression.
growth strain :
Mammalian expression of human Sumo1 with HA tag
origin :
37
pi :
alt_names
Sumo1
cloning
clone_methodRestriction Enzyme
cloning_site_3SmaI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesDAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1
geneSUMO1
id7341
genbank_ids
mutation
namepcDNA3-HA-Sumo1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
resistance markers :
607
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA