This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pmRFP-LC3
catalog :
21075
citations: 69
Reference
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Ke P, Chang C, Hsiao Y. Baicalein Activates Parkin-Dependent Mitophagy through NDP52 and OPTN. Cells. 2022;11: pubmed publisher
Xiao W, Wang J, Wang X, Cai S, Guo Y, Ye L, et al. Therapeutic targeting of the USP2-E2F4 axis inhibits autophagic machinery essential for zinc homeostasis in cancer progression. Autophagy. 2022;:1-21 pubmed publisher
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Sønder S, Häger S, Heitmann A, Frankel L, Dias C, Simonsen A, et al. Restructuring of the plasma membrane upon damage by LC3-associated macropinocytosis. Sci Adv. 2021;7: pubmed publisher
Chen K, Yan R, Xiang L, Xu K. Excitation spectral microscopy for highly multiplexed fluorescence imaging and quantitative biosensing. Light Sci Appl. 2021;10:97 pubmed publisher
Lim Y, Kim S, Kim E. Palmitate reduces starvation-induced ER stress by inhibiting ER-phagy in hypothalamic cells. Mol Brain. 2021;14:65 pubmed publisher
Chen Y, Wang R, Huang S, Henson E, Bi J, Gibson S. Erb-b2 Receptor Tyrosine Kinase 2 (ERBB2) Promotes ATG12-Dependent Autophagy Contributing to Treatment Resistance of Breast Cancer Cells. Cancers (Basel). 2021;13: pubmed publisher
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Peláez Coyotl E, Barrios Palacios J, Muciño G, Moreno Blas D, Costas M, Montiel Montes T, et al. Antimicrobial Peptide against Mycobacterium Tuberculosis That Activates Autophagy Is an Effective Treatment for Tuberculosis. Pharmaceutics. 2020;12: pubmed publisher
Mao M, Chang C, Pickar Oliver A, Cervia L, Wang L, Ji J, et al. Redirecting Vesicular Transport to Improve Nonviral Delivery of Molecular Cargo. Adv Biosyst. 2020;4:e2000059 pubmed publisher
Fang C, Woo J, Liu T, Zhao X, Cazzaro S, Yan Y, et al. SSH1 impedes SQSTM1/p62 flux and MAPT/Tau clearance independent of CFL (cofilin) activation. Autophagy. 2020;:1-22 pubmed publisher
Bressan C, Pecora A, Gagnon D, Snapyan M, Labrecque S, De Koninck P, et al. The dynamic interplay between ATP/ADP levels and autophagy sustain neuronal migration in vivo. elife. 2020;9: pubmed publisher
Bindschedler A, Wacker R, Egli J, Eickel N, Schmuckli Maurer J, Franke Fayard B, et al. Plasmodium berghei sporozoites in nonreplicative vacuole are eliminated by a PI3P-mediated autophagy-independent pathway. Cell Microbiol. 2021;23:e13271 pubmed publisher
Herbst S, Campbell P, Harvey J, Bernard E, Papayannopoulos V, Wood N, et al. LRRK2 activation controls the repair of damaged endomembranes in macrophages. EMBO J. 2020;:e104494 pubmed publisher
Elimam H, El Say K, Cybulsky A, Khalil H. Regulation of Autophagy Progress via Lysosomal Depletion by Fluvastatin Nanoparticle Treatment in Breast Cancer Cells. ACS Omega. 2020;5:15476-15486 pubmed publisher
Peña Oyarzun D, Rodriguez Peña M, Burgos Bravo F, Vergara A, Kretschmar C, Sotomayor Flores C, et al. PKD2/polycystin-2 induces autophagy by forming a complex with BECN1. Autophagy. 2020;:1-15 pubmed publisher
Shen H, Zhu H, Panja D, Gu Q, Li Z. Autophagy controls the induction and developmental decline of NMDAR-LTD through endocytic recycling. Nat Commun. 2020;11:2979 pubmed publisher
Fu R, Zhang L, Li Y, Li B, Ming Y, Li Z, et al. Saikosaponin D inhibits autophagosome‑lysosome fusion and induces autophagy‑independent apoptosis in MDA‑MB‑231 breast cancer cells. Mol Med Rep. 2020;22:1026-1034 pubmed publisher
Wang T, Li W, Martin S, Papadopulos A, Joensuu M, Liu C, et al. Radial contractility of actomyosin rings facilitates axonal trafficking and structural stability. J Cell Biol. 2020;219: pubmed publisher
Hirano S, Kanno S. Relevance of autophagy markers to cytotoxicity of zinc compounds in macrophages. Toxicol In Vitro. 2020;65:104816 pubmed publisher
Zambrano J, Eblen S, Abt M, Rhett J, Muise Helmericks R, Yeh E. HUNK Phosphorylates Rubicon to Support Autophagy. Int J Mol Sci. 2019;20: pubmed publisher
Elimam H, Papillon J, Guillemette J, Navarro Betancourt J, Cybulsky A. Genetic Ablation of Calcium-independent Phospholipase A2γ Exacerbates Glomerular Injury in Adriamycin Nephrosis in Mice. Sci Rep. 2019;9:16229 pubmed publisher
Kim S, Kim N, Park S, Jeon Y, Lee J, Yoo S, et al. Tanycytic TSPO inhibition induces lipophagy to regulate lipid metabolism and improve energy balance. Autophagy. 2019;:1-21 pubmed publisher
Fernández Mosquera L, Yambire K, Couto R, Pereyra L, Pabis K, Ponsford A, et al. Mitochondrial respiratory chain deficiency inhibits lysosomal hydrolysis. Autophagy. 2019;:1-20 pubmed publisher
Pereira G, Sanchez L, Schaughency P, Rubio Roldán A, Choi J, Planet E, et al. Properties of LINE-1 proteins and repeat element expression in the context of amyotrophic lateral sclerosis. Mob DNA. 2018;9:35 pubmed publisher
Chen X, Hu Y, Zhang W, Chen K, Hu J, Li X, et al. Cisplatin induces autophagy to enhance hepatitis B virus replication via activation of ROS/JNK and inhibition of the Akt/mTOR pathway. Free Radic Biol Med. 2019;131:225-236 pubmed publisher
Yee A, Papillon J, Guillemette J, Kaufman D, Kennedy C, Cybulsky A. Proteostasis as a Therapeutic Target in Glomerular Injury Associated with Mutant Alpha-Actinin-4. Am J Physiol Renal Physiol. 2018;: pubmed publisher
Bai Y, Liu C, Fu L, Gong X, Dou C, Cao Z, et al. Mangiferin enhances endochondral ossification-based bone repair in massive bone defect by inducing autophagy through activating AMP-activated protein kinase signaling pathway. FASEB J. 2018;32:4573-4584 pubmed publisher
Kucharewicz K, Dudkowska M, Zawadzka A, Ogrodnik M, Szczepankiewicz A, Czarnocki Z, et al. Simultaneous induction and blockade of autophagy by a single agent. Cell Death Dis. 2018;9:353 pubmed publisher
Fu R, Deng Q, Zhang H, Hu X, Li Y, Liu Y, et al. A novel autophagy inhibitor berbamine blocks SNARE-mediated autophagosome-lysosome fusion through upregulation of BNIP3. Cell Death Dis. 2018;9:243 pubmed publisher
Shinoda H, Ma Y, Nakashima R, Sakurai K, Matsuda T, Nagai T. Acid-Tolerant Monomeric GFP from Olindias formosa. Cell Chem Biol. 2018;25:330-338.e7 pubmed publisher
Lin S, Hsieh S, Fan Y, Wei W, Hsiao P, Tsai D, et al. Necroptosis promotes autophagy-dependent upregulation of DAMP and results in immunosurveillance. Autophagy. 2018;14:778-795 pubmed publisher
Sparrer K, Gableske S, Zurenski M, Parker Z, Full F, Baumgart G, et al. TRIM23 mediates virus-induced autophagy via activation of TBK1. Nat Microbiol. 2017;2:1543-1557 pubmed publisher
Schmuckli Maurer J, Reber V, Wacker R, Bindschedler A, Zakher A, Heussler V. Inverted recruitment of autophagy proteins to the Plasmodium berghei parasitophorous vacuole membrane. PLoS ONE. 2017;12:e0183797 pubmed publisher
Stewart S, Menzies S, Popa S, Savinykh N, Petrunkina Harrison A, Lehner P, et al. A genome-wide CRISPR screen reconciles the role of N-linked glycosylation in galectin-3 transport to the cell surface. J Cell Sci. 2017;130:3234-3247 pubmed publisher
Micheva Viteva S, Shou Y, Ganguly K, Wu T, Hong Geller E. PKC-η-MARCKS Signaling Promotes Intracellular Survival of Unopsonized Burkholderia thailandensis. Front Cell Infect Microbiol. 2017;7:231 pubmed publisher
Dagda R, Rice M. Protocols for assessing mitophagy in neuronal cell lines and primary neurons. Neuromethods. 2017;123:249-277 pubmed publisher
Agop Nersesian C, De Niz M, Niklaus L, Prado M, Eickel N, Heussler V. Shedding of host autophagic proteins from the parasitophorous vacuolar membrane of Plasmodium berghei. Sci Rep. 2017;7:2191 pubmed publisher
Okerlund N, Schneider K, Leal Ortiz S, Montenegro Venegas C, Kim S, Garner L, et al. Bassoon Controls Presynaptic Autophagy through Atg5. Neuron. 2017;93:897-913.e7 pubmed publisher
Ashrafi G, Wu Z, Farrell R, Ryan T. GLUT4 Mobilization Supports Energetic Demands of Active Synapses. Neuron. 2017;93:606-615.e3 pubmed publisher
Li G, Fu R, Shen H, Zhou J, Hu X, Liu Y, et al. Polyphyllin I induces mitophagic and apoptotic cell death in human breast cancer cells by increasing mitochondrial PINK1 levels. Oncotarget. 2017;8:10359-10374 pubmed publisher
Deng H, Shi Y, Yang Y, Ahmeti K, Miller N, Huang C, et al. Identification of TMEM230 mutations in familial Parkinson's disease. Nat Genet. 2016;48:733-9 pubmed publisher
Chung K, Jeong E, Park H, An H, Yu S. Mediation of Autophagic Cell Death by Type 3 Ryanodine Receptor (RyR3) in Adult Hippocampal Neural Stem Cells. Front Cell Neurosci. 2016;10:116 pubmed publisher
Chen Y, Henson E, Xiao W, Huang D, McMillan Ward E, Israels S, et al. Tyrosine kinase receptor EGFR regulates the switch in cancer cells between cell survival and cell death induced by autophagy in hypoxia. Autophagy. 2016;12:1029-46 pubmed publisher
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Liu H, Gu L, Tu Y, Hu H, Huang Y, Sun W. Emodin ameliorates cisplatin-induced apoptosis of rat renal tubular cells in vitro by activating autophagy. Acta Pharmacol Sin. 2016;37:235-45 pubmed publisher
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Wang T, Martin S, Papadopulos A, Harper C, Mavlyutov T, Niranjan D, et al. Control of autophagosome axonal retrograde flux by presynaptic activity unveiled using botulinum neurotoxin type a. J Neurosci. 2015;35:6179-94 pubmed publisher
Tamura A, Yui N. β-Cyclodextrin-threaded biocleavable polyrotaxanes ameliorate impaired autophagic flux in Niemann-Pick type C disease. J Biol Chem. 2015;290:9442-54 pubmed publisher
Toops K, Tan L, Jiang Z, Radu R, Lakkaraju A. Cholesterol-mediated activation of acid sphingomyelinase disrupts autophagy in the retinal pigment epithelium. Mol Biol Cell. 2015;26:1-14 pubmed publisher
Martin D, Heit R, Yap M, Davidson M, Hayden M, Berthiaume L. Identification of a post-translationally myristoylated autophagy-inducing domain released by caspase cleavage of huntingtin. Hum Mol Genet. 2014;23:3166-79 pubmed publisher
Liu Y, Joo K, Lei Y, Wang P. Visualization of intracellular pathways of engineered baculovirus in mammalian cells. Virus Res. 2014;181:81-91 pubmed publisher
Romao S, Gasser N, Becker A, Guhl B, Bajagic M, Vanoaica D, et al. Autophagy proteins stabilize pathogen-containing phagosomes for prolonged MHC II antigen processing. J Cell Biol. 2013;203:757-66 pubmed publisher
Delorme Axford E, Donker R, Mouillet J, Chu T, Bayer A, Ouyang Y, et al. Human placental trophoblasts confer viral resistance to recipient cells. Proc Natl Acad Sci U S A. 2013;110:12048-53 pubmed publisher
Nunes P, Ernandez T, Roth I, Qiao X, Strebel D, Bouley R, et al. Hypertonic stress promotes autophagy and microtubule-dependent autophagosomal clusters. Autophagy. 2013;9:550-67 pubmed publisher
Atkin T, Brandon N, Kittler J. Disrupted in Schizophrenia 1 forms pathological aggresomes that disrupt its function in intracellular transport. Hum Mol Genet. 2012;21:2017-28 pubmed publisher
Rivera J, Gurlo T, Daval M, Huang C, Matveyenko A, Butler P, et al. Human-IAPP disrupts the autophagy/lysosomal pathway in pancreatic ?-cells: protective role of p62-positive cytoplasmic inclusions. Cell Death Differ. 2011;18:415-26 pubmed publisher
Bhoopathi P, Chetty C, Gujrati M, Dinh D, Rao J, Lakka S. Cathepsin B facilitates autophagy-mediated apoptosis in SPARC overexpressed primitive neuroectodermal tumor cells. Cell Death Differ. 2010;17:1529-39 pubmed publisher
Kimura S, Noda T, Yoshimori T. Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3. Autophagy. 2007;3:452-60 pubmed
product information
Catalog Number :
21075
Product Name :
pmRFP-LC3
article :
doi10.4161/auto.4451
id2504
pubmed_id17534139
bacterial resistance :
Kanamycin
cloning :
backbonepmRFP-C1
backbone_mutation
backbone_origin
backbone_size4500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Compbell RE, Tour O, Palmer AE, Steinbach PA, Baird GS, Zacharian DA, Tsien RY, A monomeric red fluorescent protein, Proc Natl Acad Sci USA. 2002, 99(12):7877-82
growth strain :
Mammalian expression of rat LC3 fused to mRFP
origin :
37
pi :
alt_names
Map1lc3b
LC3
LC3b
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BglII
promoter
sequencing_primer_3
sequencing_primer_5N/A
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesLC3B, Map1lc3, Mpl3, zbs559
geneMap1lc3b
id64862
genbank_ids
U05784
mutationFirst ATG is not included.
namemicrotubule-associated protein 1 light chain 3 beta
shRNA_sequence
size738
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagmRFP
resistance markers :
603
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA