This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-LC3
catalog :
21073
citations: 77
Reference
Jang H, Lee Y, Dao T, Jo Y, Khim K, Eom H, et al. Thrap3 promotes nonalcoholic fatty liver disease by suppressing AMPK-mediated autophagy. Exp Mol Med. 2023;: pubmed publisher
Song T, Yin F, Wang Z, Zhang H, Liu P, Guo Y, et al. Hsp70-Bim interaction facilitates mitophagy by recruiting parkin and TOMM20 into a complex. Cell Mol Biol Lett. 2023;28:46 pubmed publisher
Cason S, Holzbaur E. Axonal transport of autophagosomes is regulated by dynein activators JIP3/JIP4 and ARF/RAB GTPases. bioRxiv. 2023;: pubmed publisher
Gallagher E, Holzbaur E. The selective autophagy adaptor p62/SQSTM1 forms phase condensates regulated by HSP27 that facilitate the clearance of damaged lysosomes via lysophagy. Cell Rep. 2023;42:112037 pubmed publisher
Sen A, Kallabis S, Gaedke F, J xfc ngst C, Boix J, N xfc chel J, et al. Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA. Nat Commun. 2022;13:6704 pubmed publisher
Fan Y, Zhang Y, Bahreyni A, Luo H, Mohamud Y. Coxsackievirus Protease 2A Targets Host Protease ATG4A to Impair Autophagy. Viruses. 2022;14: pubmed publisher
Cason S, Mogre S, Holzbaur E, Koslover E. Spatiotemporal analysis of axonal autophagosome-lysosome dynamics reveals limited fusion events and slow maturation. Mol Biol Cell. 2022;33:ar123 pubmed publisher
Ke P, Chang C, Hsiao Y. Baicalein Activates Parkin-Dependent Mitophagy through NDP52 and OPTN. Cells. 2022;11: pubmed publisher
Xiao W, Wang J, Wang X, Cai S, Guo Y, Ye L, et al. Therapeutic targeting of the USP2-E2F4 axis inhibits autophagic machinery essential for zinc homeostasis in cancer progression. Autophagy. 2022;:1-21 pubmed publisher
Xing Y, Wei X, Wang M, Liu Y, Sui Z, Wang X, et al. Stimulating TRPM7 suppresses cancer cell proliferation and metastasis by inhibiting autophagy. Cancer Lett. 2022;525:179-197 pubmed publisher
Xing Y, Wei X, Liu Y, Wang M, Sui Z, Wang X, et al. Autophagy inhibition mediated by MCOLN1/TRPML1 suppresses cancer metastasis via regulating a ROS-driven TP53/p53 pathway. Autophagy. 2021;:1-23 pubmed publisher
You J, Lee J, Roh J. PGRMC1-dependent lipophagy promotes ferroptosis in paclitaxel-tolerant persister cancer cells. J Exp Clin Cancer Res. 2021;40:350 pubmed publisher
Pan P, Zhu J, Rizvi A, Zhu X, Tanaka H, Dreyfus C. Synaptojanin1 deficiency upregulates basal autophagosome formation in astrocytes. J Biol Chem. 2021;297:100873 pubmed publisher
Qi J, Xing Y, Liu Y, Wang M, Wei X, Sui Z, et al. MCOLN1/TRPML1 finely controls oncogenic autophagy in cancer by mediating zinc influx. Autophagy. 2021;:1-22 pubmed publisher
Rigby M, Lawton A, Kaur G, Banduseela V, Kamm W, Lakkaraju A, et al. Endoplasmic reticulum acetyltransferases Atase1 and Atase2 differentially regulate reticulophagy, macroautophagy and cellular acetyl-CoA metabolism. Commun Biol. 2021;4:454 pubmed publisher
Heacock Kang Y, McMillan I, Norris M, Sun Z, Zarzycki Siek J, Bluhm A, et al. The Burkholderia pseudomallei intracellular 'TRANSITome'. Nat Commun. 2021;12:1907 pubmed publisher
Baek M, Choe Y, Bannwarth S, Kim J, Maitra S, Dorn G, et al. TDP-43 and PINK1 mediate CHCHD10S59L mutation-induced defects in Drosophila and in vitro. Nat Commun. 2021;12:1924 pubmed publisher
Zhou J, Singh B, Ho J, Lim A, Bruinstroop E, Ohba K, et al. MED1 mediator subunit is a key regulator of hepatic autophagy and lipid metabolism. Autophagy. 2021;:1-19 pubmed publisher
Ryu H, Kim L, Jeong H, Yeo B, Lee J, Nam H, et al. GSK3B induces autophagy by phosphorylating ULK1. Exp Mol Med. 2021;53:369-383 pubmed publisher
Huang X, Huang J, Du J, Zhang N, Long Z, Yang Y, et al. Autophagy inhibitors increase the susceptibility of KRAS-mutant human colorectal cancer cells to a combined treatment of 2-deoxy-D-glucose and lovastatin. Acta Pharmacol Sin. 2021;: pubmed publisher
Dai W, Wang M, Wang P, Wen J, Wang J, Cha S, et al. lncRNA NEAT1 ameliorates LPS‑induced inflammation in MG63 cells by activating autophagy and suppressing the NLRP3 inflammasome. Int J Mol Med. 2021;47:607-620 pubmed publisher
Inomata M, Xu S, Chandra P, Meydani S, Takemura G, Philips J, et al. Macrophage LC3-associated phagocytosis is an immune defense against Streptococcus pneumoniae that diminishes with host aging. Proc Natl Acad Sci U S A. 2020;117:33561-33569 pubmed publisher
Cantarero L, Juárez Escoto E, Civera Tregón A, Rodríguez Sanz M, Roldán M, Benitez R, et al. Mitochondria-lysosome membrane contacts are defective in GDAP1-related Charcot-Marie-Tooth disease. Hum Mol Genet. 2021;29:3589-3605 pubmed publisher
Rao L, De La Rosa I, Xu Y, Sha Y, Bhattacharya A, Holtzman M, et al. Pseudomonas aeruginosa survives in epithelia by ExoS-mediated inhibition of autophagy and mTOR. EMBO Rep. 2021;22:e50613 pubmed publisher
Kobayashi M, Yasukawa H, Arikawa T, Deguchi Y, Mizushima N, Sakurai M, et al. Trehalose induces SQSTM1/p62 expression and enhances lysosomal activity and antioxidative capacity in adipocytes. FEBS Open Bio. 2020;: pubmed publisher
Suma P, Padmanabhan R, Telukutla S, Ravindran R, Velikkakath A, Dekiwadia C, et al. Vanadium pentoxide nanoparticle mediated perturbations in cellular redox balance and the paradigm of autophagy to apoptosis. Free Radic Biol Med. 2020;161:198-211 pubmed publisher
Ming K, He M, Su L, Du H, Wang D, Wu Y, et al. The inhibitory effect of phosphorylated Codonopsis pilosula polysaccharide on autophagosomes formation contributes to the inhibition of duck hepatitis A virus replication. Poult Sci. 2020;99:2146-2156 pubmed publisher
Thayyullathil F, Cheratta A, Pallichankandy S, Subburayan K, Tariq S, Rangnekar V, et al. Par-4 regulates autophagic cell death in human cancer cells via upregulating p53 and BNIP3. Biochim Biophys Acta Mol Cell Res. 2020;1867:118692 pubmed publisher
Machihara K, Namba T. BAP31 Inhibits Cell Adaptation to ER Stress Conditions, Negatively Regulating Autophagy Induction by Interaction with STX17. Cells. 2019;8: pubmed publisher
Denu R, Kaur G, Sass M, Lakkaraju A, Burkard M. Centrosome Amplification in Cancer Disrupts Autophagy and Sensitizes to Autophagy Inhibition. Mol Cancer Res. 2019;: pubmed publisher
Ávila Pérez G, Diaz Beneitez E, Cubas Gaona L, Nieves Molina G, Rodríguez J, Rodriguez J, et al. Activation of the autophagy pathway by Torovirus infection is irrelevant for virus replication. PLoS ONE. 2019;14:e0219428 pubmed publisher
Chaurasia M, Gupta S, Das A, Dwarakanath B, Simonsen A, Sharma K. Radiation induces EIF2AK3/PERK and ERN1/IRE1 mediated pro-survival autophagy. Autophagy. 2019;15:1391-1406 pubmed publisher
Zahedi A, On V, Phandthong R, Chaili A, Remark G, Bhanu B, et al. Deep Analysis of Mitochondria and Cell Health Using Machine Learning. Sci Rep. 2018;8:16354 pubmed publisher
Yau W, Singh B, Lesmana R, Zhou J, Sinha R, Wong K, et al. Thyroid hormone (T3) stimulates brown adipose tissue activation via mitochondrial biogenesis and MTOR-mediated mitophagy. Autophagy. 2019;15:131-150 pubmed publisher
Tang Y, Blomenkamp K, Fickert P, Trauner M, Teckman J. NorUDCA promotes degradation of α1-antitrypsin mutant Z protein by inducing autophagy through AMPK/ULK1 pathway. PLoS ONE. 2018;13:e0200897 pubmed publisher
Sekar S, Wu X, Friis T, Crawford R, Prasadam I, Xiao Y. Saturated fatty acids promote chondrocyte matrix remodeling through reprogramming of autophagy pathways. Nutrition. 2018;54:144-152 pubmed publisher
Duan X, Kong Z, Mai X, Lan Y, Liu Y, Yang Z, et al. Autophagy inhibition attenuates hyperoxaluria-induced renal tubular oxidative injury and calcium oxalate crystal depositions in the rat kidney. Redox Biol. 2018;16:414-425 pubmed publisher
Kaneko H, Kobayashi M, Mizunoe Y, Yoshida M, Yasukawa H, Hoshino S, et al. Taurine is an amino acid with the ability to activate autophagy in adipocytes. Amino Acids. 2018;50:527-535 pubmed publisher
Singh N, Kansal P, Ahmad Z, Baid N, Kushwaha H, Khatri N, et al. Antimycobacterial effect of IFNG (interferon gamma)-induced autophagy depends on HMOX1 (heme oxygenase 1)-mediated increase in intracellular calcium levels and modulation of PPP3/calcineurin-TFEB (transcription factor EB) axis. Autophagy. 2018;14:972-991 pubmed publisher
Liu P, Tsai K, Hsu C, Tsai W, Cheng J, Chang H, et al. Drug Repurposing Screening Identifies Tioconazole as an ATG4 Inhibitor that Suppresses Autophagy and Sensitizes Cancer Cells to Chemotherapy. Theranostics. 2018;8:830-845 pubmed publisher
Kang Y, Hsu W, Wu C, Hsin I, Wu P, Yeh K, et al. Metformin alleviates nickel-induced autophagy and apoptosis via inhibition of hexokinase-2, activating lipocalin-2, in human bronchial epithelial cells. Oncotarget. 2017;8:105536-105552 pubmed publisher
Mizunoe Y, Kobayashi M, Sudo Y, Watanabe S, Yasukawa H, Natori D, et al. Trehalose protects against oxidative stress by regulating the Keap1-Nrf2 and autophagy pathways. Redox Biol. 2018;15:115-124 pubmed publisher
Singh B, Saqib M, Gupta A, Kumar P, Bhaskar S. Autophagy induction by Mycobacterium indicus pranii promotes Mycobacterium tuberculosis clearance from RAW 264.7 macrophages. PLoS ONE. 2017;12:e0189606 pubmed publisher
Lin S, Hsieh S, Fan Y, Wei W, Hsiao P, Tsai D, et al. Necroptosis promotes autophagy-dependent upregulation of DAMP and results in immunosurveillance. Autophagy. 2018;14:778-795 pubmed publisher
Xiao B, Deng X, Lim G, Xie S, Zhou Z, Lim K, et al. Superoxide drives progression of Parkin/PINK1-dependent mitophagy following translocation of Parkin to mitochondria. Cell Death Dis. 2017;8:e3097 pubmed publisher
Xiao B, Goh J, Xiao L, Xian H, Lim K, Liou Y. Reactive oxygen species trigger Parkin/PINK1 pathway-dependent mitophagy by inducing mitochondrial recruitment of Parkin. J Biol Chem. 2017;292:16697-16708 pubmed publisher
Ha S, Jeong S, Yi K, Chung K, Hong C, Kim S, et al. Phosphorylation of p62 by AMP-activated protein kinase mediates autophagic cell death in adult hippocampal neural stem cells. J Biol Chem. 2017;292:13795-13808 pubmed publisher
Kim H, Joe Y, Kim S, Park S, Park J, Chen Y, et al. Carbon monoxide protects against hepatic steatosis in mice by inducing sestrin-2 via the PERK-eIF2?-ATF4 pathway. Free Radic Biol Med. 2017;110:81-91 pubmed publisher
Okerlund N, Schneider K, Leal Ortiz S, Montenegro Venegas C, Kim S, Garner L, et al. Bassoon Controls Presynaptic Autophagy through Atg5. Neuron. 2017;93:897-913.e7 pubmed publisher
Fan T, Chen L, Huang Z, Wang W, Zhang B, Xu Y, et al. Autophagy Activation by Rapamycin Before Hypoxia-Reoxygenation Reduces Endoplasmic Reticulum Stress in Alveolar Epithelial Cells. Cell Physiol Biochem. 2017;41:79-90 pubmed publisher
Moriyama M, Moriyama H, Uda J, Kubo H, Nakajima Y, Goto A, et al. BNIP3 upregulation via stimulation of ERK and JNK activity is required for the protection of keratinocytes from UVB-induced apoptosis. Cell Death Dis. 2017;8:e2576 pubmed publisher
Li D, Hu J, Wang T, Zhang X, Liu L, Wang H, et al. Silymarin attenuates cigarette smoke extract-induced inflammation via simultaneous inhibition of autophagy and ERK/p38 MAPK pathway in human bronchial epithelial cells. Sci Rep. 2016;6:37751 pubmed publisher
Hubert V, Peschel A, Langer B, Groger M, Rees A, Kain R. LAMP-2 is required for incorporating syntaxin-17 into autophagosomes and for their fusion with lysosomes. Biol Open. 2016;5:1516-1529 pubmed publisher
Kim M, Bae S, Ryu J, Kwon Y, Oh J, Kwon J, et al. SESN2/sestrin2 suppresses sepsis by inducing mitophagy and inhibiting NLRP3 activation in macrophages. Autophagy. 2016;12:1272-91 pubmed publisher
Chung K, Jeong E, Park H, An H, Yu S. Mediation of Autophagic Cell Death by Type 3 Ryanodine Receptor (RyR3) in Adult Hippocampal Neural Stem Cells. Front Cell Neurosci. 2016;10:116 pubmed publisher
Shao J, Yang X, Liu T, Zhang T, Xie Q, Xia W. Autophagy induction by SIRT6 is involved in oxidative stress-induced neuronal damage. Protein Cell. 2016;7:281-290 pubmed publisher
Seggewiß N, Kruse H, Weilandt R, Domsgen E, Dotzauer A, Paulmann D. Cellular localization and effects of ectopically expressed hepatitis A virus proteins 2B, 2C, 3A and their intermediates 2BC, 3AB and 3ABC. Arch Virol. 2016;161:851-65 pubmed publisher
Korah J, Canaff L, Lebrun J. The Retinoblastoma Tumor Suppressor Protein (pRb)/E2 Promoter Binding Factor 1 (E2F1) Pathway as a Novel Mediator of TGFβ-induced Autophagy. J Biol Chem. 2016;291:2043-54 pubmed publisher
Farah B, Landau D, Sinha R, Brooks E, Wu Y, Fung S, et al. Induction of autophagy improves hepatic lipid metabolism in glucose-6-phosphatase deficiency. J Hepatol. 2016;64:370-379 pubmed publisher
Nishikawa Y, Okuzaki D, Fukushima K, Mukai S, Ohno S, Ozaki Y, et al. Withaferin A Induces Cell Death Selectively in Androgen-Independent Prostate Cancer Cells but Not in Normal Fibroblast Cells. PLoS ONE. 2015;10:e0134137 pubmed publisher
Li Y, Li R, Zhu S, Zhou R, Wang L, Du J, et al. Cordycepin induces apoptosis and autophagy in human neuroblastoma SK-N-SH and BE(2)-M17 cells. Oncol Lett. 2015;9:2541-2547 pubmed
Sinha R, Singh B, Zhou J, Wu Y, Farah B, Ohba K, et al. Thyroid hormone induction of mitochondrial activity is coupled to mitophagy via ROS-AMPK-ULK1 signaling. Autophagy. 2015;11:1341-57 pubmed publisher
Wang T, Martin S, Papadopulos A, Harper C, Mavlyutov T, Niranjan D, et al. Control of autophagosome axonal retrograde flux by presynaptic activity unveiled using botulinum neurotoxin type a. J Neurosci. 2015;35:6179-94 pubmed publisher
Girón M, Vílchez J, Shreeram S, Salto R, Manzano M, Cabrera E, et al. β-Hydroxy-β-methylbutyrate (HMB) normalizes dexamethasone-induced autophagy-lysosomal pathway in skeletal muscle. PLoS ONE. 2015;10:e0117520 pubmed publisher
Liang L, Ma B, Liang Y, Liu H, Zhang T, Zheng G, et al. Obatoclax induces Beclin 1- and ATG5-dependent apoptosis and autophagy in adenoid cystic carcinoma cells. Oral Dis. 2015;21:470-7 pubmed publisher
Toops K, Tan L, Jiang Z, Radu R, Lakkaraju A. Cholesterol-mediated activation of acid sphingomyelinase disrupts autophagy in the retinal pigment epithelium. Mol Biol Cell. 2015;26:1-14 pubmed publisher
Thayyullathil F, Rahman A, Pallichankandy S, Patel M, Galadari S. ROS-dependent prostate apoptosis response-4 (Par-4) up-regulation and ceramide generation are the prime signaling events associated with curcumin-induced autophagic cell death in human malignant glioma. FEBS Open Bio. 2014;4:763-76 pubmed publisher
Farah B, Sinha R, Wu Y, Singh B, Zhou J, Bay B, et al. ?-Adrenergic agonist and antagonist regulation of autophagy in HepG2 cells, primary mouse hepatocytes, and mouse liver. PLoS ONE. 2014;9:e98155 pubmed publisher
Salto R, Vílchez J, Cabrera E, Guinovart J, Girón M. Activation of ERK by sodium tungstate induces protein synthesis and prevents protein degradation in rat L6 myotubes. FEBS Lett. 2014;588:2246-54 pubmed publisher
Nakamura O, Hitora T, Yamagami Y, Mori M, Nishimura H, Horie R, et al. The combination of rapamycin and MAPK inhibitors enhances the growth inhibitory effect on Nara-H cells. Int J Mol Med. 2014;33:1491-7 pubmed publisher
Zhou J, Farah B, Sinha R, Wu Y, Singh B, Bay B, et al. Epigallocatechin-3-gallate (EGCG), a green tea polyphenol, stimulates hepatic autophagy and lipid clearance. PLoS ONE. 2014;9:e87161 pubmed publisher
Santiago F, Covaleda L, Sanchez Aparicio M, Silvas J, Diaz Vizarreta A, Patel J, et al. Hijacking of RIG-I signaling proteins into virus-induced cytoplasmic structures correlates with the inhibition of type I interferon responses. J Virol. 2014;88:4572-85 pubmed publisher
Moriyama M, Moriyama H, Uda J, Matsuyama A, Osawa M, Hayakawa T. BNIP3 plays crucial roles in the differentiation and maintenance of epidermal keratinocytes. J Invest Dermatol. 2014;134:1627-1635 pubmed publisher
Jang B, Choi B, Kim J, Kim M, Sohn M, Suh S. Impairment of autophagic flux promotes glucose reperfusion-induced neuro2A cell death after glucose deprivation. PLoS ONE. 2013;8:e76466 pubmed publisher
Steele S, Brunton J, Ziehr B, Taft Benz S, Moorman N, KAWULA T. Francisella tularensis harvests nutrients derived via ATG5-independent autophagy to support intracellular growth. PLoS Pathog. 2013;9:e1003562 pubmed publisher
Sinha R, Farah B, Singh B, Siddique M, Li Y, Wu Y, et al. Caffeine stimulates hepatic lipid metabolism by the autophagy-lysosomal pathway in mice. Hepatology. 2014;59:1366-80 pubmed publisher
Kabeya Y, Mizushima N, Ueno T, Yamamoto A, Kirisako T, Noda T, et al. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J. 2000;19:5720-8 pubmed
product information
Catalog Number :
21073
Product Name :
pEGFP-LC3
article :
doi10.1093/emboj/19.21.5720
id2565
pubmed_id11060023
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originclontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Mammalian expression of rat LC3 fused to EGFP
origin :
37
pi :
alt_names
Map1lc3b
LC3
LC3b
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BglII
promoterCMV
sequencing_primer_3SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesLC3B, Map1lc3, Mpl3, zbs559
geneMap1lc3b
id64862
genbank_ids
U05784
mutationFirst ATG is not included.
namemicrotubule-associated protein 1 light chain 3 beta
shRNA_sequence
size738
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagEGFP
resistance markers :
603
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA