RCAS(B)-Rheb-Q64L | Addgene 21050 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

RCAS(B)-Rheb-Q64L

catalog :

21050

citations: 2

Reference
Benito Cuesta I, Ordóñez Gutiérrez L, Wandosell F. AMPK activation does not enhance autophagy in neurons in contrast to MTORC1 inhibition: different impact on β-amyloid clearance. Autophagy. 2020;:1-16 pubmed publisher
Jiang H, Vogt P. Constitutively active Rheb induces oncogenic transformation. Oncogene. 2008;27:5729-40 pubmed publisher

product information

Catalog Number :

21050

Product Name :

RCAS(B)-Rheb-Q64L

article :

doi	10.1038/onc.2008.180
id	2429
pubmed_id	18521078

bacterial resistance :

Ampicillin

cloning :

backbone

pBR322

backbone_mutation

backbone_origin

backbone_size

11689

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

Retroviral

growth notes :

The creation of RCAS(B)-SfiI and the adapter plasmid pBSFI was described in Aoki et al., PNAS. 1998 Dec 8;95(25):14950-5. The SacII/XbaI fragment was cloned into pBSFI and the Sfi fragment was further cloned into RCAS(B)-SfiI.

origin :

pi :

64 to leucinenameRas-homolog enriched in brainshRNA_sequencesize685species

9606

Homo sapiens

tags

location	N terminal on insert
tag	FLAG

Enzymecloning_site_3<
/td>SfiI(B)clonin
g_site_5SfiI(A)<
td>promoters
equencing_primer_3r>sequencing_primer_5AGCTC
CGCGGGCCACCATGGACTACAAGGATGACGATGACAAGG
CGGCCGCGCCGCAGTCCAAGs
ite_3_destroyed<
td>site_5_destroyed<
/table>entrez_gened>able>

ali ases	RHEB2
gen e	RHEB
id	6009

genbank_ids

table>d>glutamine

AF493921

mutation

alt_names

Rheb

cloning

clone_method

Restriction

resistance markers :

554

tags :

High Copy