This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
HOXA9-micro-MSCV
catalog :
20976
citations: 1
Reference
Shen W, Hu Y, Uttarwar L, Passegue E, Largman C. MicroRNA-126 regulates HOXA9 by binding to the homeobox. Mol Cell Biol. 2008;28:4609-19 pubmed publisher
product information
Catalog Number :
20976
Product Name :
HOXA9-micro-MSCV
article :
doi10.1128/MCB.01652-07
id2626
pubmed_id18474618
bacterial resistance :
Ampicillin
cloning :
backboneMSCV-PGK-GFP
backbone_mutation
backbone_origin
backbone_size6500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
The nucleotide sequence should not change the aa sequence. Has ribosomal recognition site (ACC) in front of the ATG protein coding start site.
ctggagttagagaaggagtttctgtttaacatgtattta
acacgggaccgcagatatgag
Mutant seq for all 3 miRNa sites is 5'-
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5MSCV
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesD6a9, Hox-1.7
geneHoxa9
id15405
genbank_ids
mutationMutations to remove binding sites for miR-145, let-7, and miR-126 in homeobox.
nameHOXA9
shRNA_sequence
size1000
species
10090
Mus musculus
tags
plasmid copy :
Debbie Wolgemuth
resistance markers :
271
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA