This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
H2B-mCherry
catalog :
20972
citations: 69
Reference
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Kim J, Song H, Austin J, Cheng W. Optimized Infectivity of the Cell-Free Single-Cycle Human Immunodeficiency Viruses Type 1 (HIV-1) and Its Restriction by Host Cells. PLoS ONE. 2013;8:e67170 pubmed publisher
Mendez Vidal C, Gámez Del Estal M, Moreno Mateos M, Espina Zambrano A, Torres B, Pintor Toro J. PTTG2 silencing results in induction of epithelial-to-mesenchymal transition and apoptosis. Cell Death Dis. 2013;4:e530 pubmed publisher
Hinde E, Cardarelli F, Digman M, Gratton E. Changes in chromatin compaction during the cell cycle revealed by micrometer-scale measurement of molecular flow in the nucleus. Biophys J. 2012;102:691-7 pubmed publisher
Lam B, Anthony E, Hordijk P. Analysis of nucleo-cytoplasmic shuttling of the proto-oncogene SET/I2PP2A. Cytometry A. 2012;81:81-9 pubmed publisher
Daum J, Potapova T, Sivakumar S, Daniel J, Flynn J, Rankin S, et al. Cohesion fatigue induces chromatid separation in cells delayed at metaphase. Curr Biol. 2011;21:1018-24 pubmed publisher
Hinde E, Cardarelli F, Digman M, Kershner A, Kimble J, Gratton E. The impact of mitotic versus interphase chromatin architecture on the molecular flow of EGFP by pair correlation analysis. Biophys J. 2011;100:1829-36 pubmed publisher
Nam H, Benezra R. High levels of Id1 expression define B1 type adult neural stem cells. Cell Stem Cell. 2009;5:515-26 pubmed publisher
product information
Catalog Number :
20972
Product Name :
H2B-mCherry
article :
doi10.1016/j.stem.2009.08.017
id2996
pubmed_id19896442
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_origin
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The accession number for H2B.1 sequence X57127 was obtained from Fraser et al., 2005 (https://doi.org/10.1002/gene.20139). The actual DNA was PCR amplified from HEK293 genomic DNA then cloned into pEGFP-N1 as described in Kanda et al., 1998 (https://doi.org/10.1016/s0960-9822(98)70156-3). That H2B.1-EGFP construct was used as template to synthesize the construct here. An EcoRI site in the H2B.1 sequence was silently mutated. The mCherry cDNA was obtained from Dr. Roger Tsien.
growth strain :
H2B fused to mCherry for chromatin visualization
origin :
37
pi :
alt_names
H2B.1
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5KpnI
promoter
sequencing_primer_3Sp6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesH2B.1, H2B/f, H2BFF, HIST1H2BB
geneH2BC3
id3018
genbank_ids
NM_021062
mutation
nameHistone cluster 1, H2bb
shRNA_sequence
size1100
species
9606
Homo sapiens
tags
locationC terminal on insert
tagmCherry
resistance markers :
25
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA