This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
H2B-Venus
catalog :
20971
citations: 5
Reference
Salgado M, Demina I, Maity P, Nagchowdhury A, Caputo A, Krol E, et al. Legume NCRs and nodule-specific defensins of actinorhizal plants-Do they share a common origin?. PLoS ONE. 2022;17:e0268683 pubmed publisher
Castillo Badillo J, Gautam N. An optogenetic model reveals cell shape regulation through FAK and fascin. J Cell Sci. 2021;134: pubmed publisher
Ilina E, Kiryushkin A, Semenova V, Demchenko N, Pawlowski K, Demchenko K. Lateral root initiation and formation within the parental root meristem of Cucurbita pepo: is auxin a key player?. Ann Bot. 2018;122:873-888 pubmed publisher
Penzo Méndez A, Chen Y, Li J, Witze E, Stanger B. Spontaneous Cell Competition in Immortalized Mammalian Cell Lines. PLoS ONE. 2015;10:e0132437 pubmed publisher
Nam H, Benezra R. High levels of Id1 expression define B1 type adult neural stem cells. Cell Stem Cell. 2009;5:515-26 pubmed publisher
product information
Catalog Number :
20971
Product Name :
H2B-Venus
article :
doi10.1016/j.stem.2009.08.017
id2996
pubmed_id19896442
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_origin
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The accession number for H2B.1 sequence X57127 was obtained from Fraser et al., 2005 (https://doi.org/10.1002/gene.20139). The actual DNA was PCR amplified from HEK293 genomic DNA then cloned into pEGFP-N1 as described in Kanda et al., 1998 (https://doi.org/10.1016/s0960-9822(98)70156-3). That H2B.1-EGFP construct was used as template to synthesize the construct here. An EcoRI site in the H2B.1 sequence was silently mutated. The Venus cDNA was obtained from Dr. Anna-Katerina Hadjantonakis.
origin :
37
pi :
alt_names
H2B.1
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5KpnI
promoter
sequencing_primer_3Sp6
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesH2B.1, H2B/f, H2BFF, HIST1H2BB
geneH2BC3
id3018
genbank_ids
NM_021062
mutation
nameHistone cluster 1, H2bb
shRNA_sequence
size400
species
9606
Homo sapiens
tags
locationC terminal on insert
tagVenus
resistance markers :
25
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA