H2B-Orange | Addgene 20969 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

H2B-Orange

catalog :

20969

citations: 1

Reference
Nam H, Benezra R. High levels of Id1 expression define B1 type adult neural stem cells. Cell Stem Cell. 2009;5:515-26 pubmed publisher

product information

Catalog Number :

20969

Product Name :

H2B-Orange

article :

doi	10.1016/j.stem.2009.08.017
id	2996
pubmed_id	19896442

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

backbone_size

5400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The accession number for H2B.1 sequence X57127 was obtained from Fraser et al., 2005 (https://doi.org/10.1002/gene.20139). The actual DNA was PCR amplified from HEK293 genomic DNA then cloned into pEGFP-N1 as described in Kanda et al., 1998 (https://doi.org/10.1016/s0960-9822(98)70156-3). That H2B.1-EGFP construct was used as template to synthesize the construct here. An EcoRI site in the H2B.1 sequence was silently mutated. The Orange cDNA was obtained from Dr. Roger Tsien.

origin :

pi :

alt_names

H2B.1

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	KpnI
promoter
sequencing_primer_3	Sp6
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	H2B.1, H2B/f, H2BFF, HIST1H2BB
gene	H2BC3
id	3018

genbank_ids

NM_021062

mutation

name

Histone cluster 1, H2bb

shRNA_sequence

size

400

species

9606

Homo sapiens