This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1/hChR2(H134R)-mCherry
catalog :
20938
citations: 17
Reference
Das S, Lituma P, Castillo P, Singer R. Maintenance of a short-lived protein required for long-term memory involves cycles of transcription and local translation. Neuron. 2023;111:2051-2064.e6 pubmed publisher
Hori S, Mitani S. The transcription factor unc-130/FOXD3/4 contributes to the biphasic calcium response required to optimize avoidance behavior. Sci Rep. 2022;12:1907 pubmed publisher
Funken M, Bruegmann T, Sasse P. Selective optogenetic stimulation of fibroblasts enables quantification of hetero-cellular coupling to cardiomyocytes in a three-dimensional model of heart tissue. Europace. 2020;22:1590-1599 pubmed publisher
Bonzanni M, Rouleau N, Levin M, Kaplan D. Optogenetically induced cellular habituation in non-neuronal cells. PLoS ONE. 2020;15:e0227230 pubmed publisher
Westergard T, McAvoy K, Russell K, Wen X, Pang Y, Morris B, et al. Repeat-associated non-AUG translation in C9orf72-ALS/FTD is driven by neuronal excitation and stress. EMBO Mol Med. 2019;11: pubmed publisher
Hori S, Oda S, Suehiro Y, Iino Y, Mitani S. OFF-responses of interneurons optimize avoidance behaviors depending on stimulus strength via electrical synapses. PLoS Genet. 2018;14:e1007477 pubmed publisher
Montgomery J, Wahlstrom Helgren S, Wiggin T, Corwin B, Lillesaar C, Masino M. Intraspinal serotonergic signaling suppresses locomotor activity in larval zebrafish. Dev Neurobiol. 2018;: pubmed publisher
Eckermann K, Ahmed H, KaramiNejadRanjbar M, Dippel S, Ogaugwu C, Kitzmann P, et al. Hyperactive piggyBac transposase improves transformation efficiency in diverse insect species. Insect Biochem Mol Biol. 2018;98:16-24 pubmed publisher
Yadav K, Chou A, Ulaganathan R, Gao H, Lee H, Pan C, et al. Targeted and efficient activation of channelrhodopsins expressed in living cells via specifically-bound upconversion nanoparticles. Nanoscale. 2017;9:9457-9466 pubmed publisher
Tanimura A, Lim S, Aceves Buendia J, Goldberg J, Surmeier D. Cholinergic Interneurons Amplify Corticostriatal Synaptic Responses in the Q175 Model of Huntington's Disease. Front Syst Neurosci. 2016;10:102 pubmed publisher
Stein B, Alonso M, Zufall F, Leinders Zufall T, Chamero P. Functional Overexpression of Vomeronasal Receptors Using a Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon. PLoS ONE. 2016;11:e0156092 pubmed publisher
Richter C, Christoph J, Lehnart S, Luther S. Optogenetic Light Crafting Tools for the Control of Cardiac Arrhythmias. Methods Mol Biol. 2016;1408:293-302 pubmed publisher
Wu T, Ramamoorthy S, Wilson T, Chen F, Porsov E, Subhash H, et al. Optogenetic Control of Mouse Outer Hair Cells. Biophys J. 2016;110:493-502 pubmed publisher
Plotkin J, Day M, Peterson J, Xie Z, Kress G, Rafalovich I, et al. Impaired TrkB receptor signaling underlies corticostriatal dysfunction in Huntington's disease. Neuron. 2014;83:178-88 pubmed publisher
Aschauer D, Kreuz S, Rumpel S. Analysis of transduction efficiency, tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse brain. PLoS ONE. 2013;8:e76310 pubmed publisher
Pham E, Mills E, Truong K. A synthetic photoactivated protein to generate local or global Ca(2+) signals. Chem Biol. 2011;18:880-90 pubmed publisher
Zhang F, Wang L, Brauner M, Liewald J, Kay K, Watzke N, et al. Multimodal fast optical interrogation of neural circuitry. Nature. 2007;446:633-9 pubmed
product information
Catalog Number :
20938
Product Name :
pcDNA3.1/hChR2(H134R)-mCherry
article :
doi10.1038/nature05744
id2540
pubmed_id17410168
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_origin
backbone_size5545
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note that the depositor's sequence is missing the H134R mutation, however Addgene's quality control sequence confirmed this mutation. The H134R mutation present in the ChR2 sequence is a gain-of-function mutant that produces larger stationary photocurrents in comparison to the wild-type ChR2 sequence
growth strain :
Mammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation
origin :
37
pi :
alt_names
ChR2
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationH134R mutation in ChR2
namechannelrhodopsin-2
shRNA_sequence
size1647
species
C. reinhardtii
tags
resistance markers :
111
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA