This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLKO shLuc/FLAG Metap2
catalog :
209316
citations: 1
Reference
Shin H, Citron Y, Wang L, Tribouillard L, Goul C, Stipp R, et al. Lysosomal GPCR-like protein LYCHOS signals cholesterol sufficiency to mTORC1. Science. 2022;377:1290-1298 pubmed publisher
product information
Catalog Number :
209316
Product Name :
pLKO shLuc/FLAG Metap2
article :
doi10.1126/science.abg6621
id28234070
pubmed_id36007018
bacterial resistance :
Ampicillin
cloning :
backbonepLKO.1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Lentiviral construct expressing FLAG Metap2 and shRNA against Luciferase (control shRNA)
origin :
37
pi :
alt_names
MAP2
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5BamHI
promoterPGK
sequencing_primer_3
sequencing_primer_5GTGTTCCGCATTCTGCAAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMAP2, MNPEP, p67eIF2
geneMETAP2
id10988
genbank_ids
mutation
nameMETAP2
shRNA_sequence
size
species
9606
Homo sapiens
32644
Other
human
tags
locationN terminal on insert
tagFlag
resistance markers :
4004
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA