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product summary
company name :
Addgene
product type :
cDNA
product name :
pEP4 E02S ET2K
catalog :
20927
citations: 46
Reference
Zeng S, Yang H, Wang B, Xie Y, Xu K, Liu L, et al. The MORC2 p.S87L mutation reduces proliferation of pluripotent stem cells derived from a patient with the spinal muscular atrophy-like phenotype by inhibiting proliferation-related signaling pathways. Neural Regen Res. 2024;19:205-211 pubmed publisher
Singh A, Singh S, Kumar M, Sarma D, Singh S, Verma V. Establishment of Capra hircus somatic cells and induction of pluripotent stem-like cells. In Vitro Cell Dev Biol Anim. 2023;: pubmed publisher
Hedges E, Cocks G, Shaw C, Nishimura A. Generation of an Open-Access Patient-Derived iPSC Biobank for Amyotrophic Lateral Sclerosis Disease Modelling. Genes (Basel). 2023;14: pubmed publisher
Baik J, Ortiz Cordero C, Magli A, Azzag K, Crist S, Yamashita A, et al. Establishment of Skeletal Myogenic Progenitors from Non-Human Primate Induced Pluripotent Stem Cells. Cells. 2023;12: pubmed publisher
Qiu S, Zhang X, Zhang L, Liu Z, Wang L, Jin Z, et al. Generation of the induced pluripotent stem cell line SFMUi001-A from a patient with usher syndrome type 2 caused by biallelic variants in the USH2A gene. Stem Cell Res. 2023;69:103101 pubmed publisher
Pourtoy Brasselet S, Sciauvaud A, Boza Morán M, Cailleret M, Jarrige M, Polvèche H, et al. Human iPSC-derived neurons reveal early developmental alteration of neurite outgrowth in the late-occurring neurodegenerative Wolfram syndrome. Am J Hum Genet. 2021;108:2171-2185 pubmed publisher
He L, Han X, Zhao H, Li S, Chen Z, Wang C, et al. Generation of spinocerebellar ataxia type 3 patient-derived induced pluripotent stem cell line (CSUXHi005-A) from human urine epithelial cells. Stem Cell Res. 2021;53:102289 pubmed publisher
Hedges E, Topp S, Shaw C, Nishimura A. Generation of six induced pluripotent stem cell lines from patients with amyotrophic lateral sclerosis with associated genetic mutations in either FUS or ANXA11. Stem Cell Res. 2021;52:102246 pubmed publisher
Rabinski T, Sagiv S, Hausman Kedem M, Fattal Valevski A, Rubinstein M, Avraham K, et al. Reprogramming of two induced pluripotent stem cell lines from a heterozygous GRIN2D developmental and epileptic encephalopathy (DEE) patient (BGUi011-A) and from a healthy family relative (BGUi012-A). Stem Cell Res. 2021;51:102178 pubmed publisher
Jiao H, Walczak B, Lee M, Lemieux M, Li W. GATA6 regulates aging of human mesenchymal stem/stromal cells. Stem Cells. 2021;39:62-77 pubmed publisher
Franz M, Hagenau L, Koch R, Neubauer S, Nowack B, Tzvetkova A, et al. Generation of an iPSC line (UMGWi001-B) from a patient with Floating-Harbor Syndrome (FLHS) carrying a heterozygous SRCAP mutation (p.Arg2444). Stem Cell Res. 2020;49:102028 pubmed publisher
Zlotnik D, Rabinski T, Ofir R, Hershkovitz E, Vatine G. Generation of iPSC lines from two (BGUi002-A and BGUi003-A) homozygous p450 oxidoreductase-deficient patients and from one (BGUi001-A) heterozygous healthy family relative. Stem Cell Res. 2020;48:101975 pubmed publisher
Falik D, Rabinski T, Zlotnik D, Eshel R, Zorsky M, Garin Shkolnik T, et al. Generation and characterization of iPSC lines (BGUi004-A, BGUi005-A) from two identical twins with polyalanine expansion in the paired-like homeobox 2B (PHOX2B) gene. Stem Cell Res. 2020;48:101955 pubmed publisher
Wei L, Zhang J, Chen D, Feng L, Wu C, Wang R, et al. Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi005-A) from a Wilson's disease patient harboring a homozygous Pro992Leu mutation in ATP7B gene. Stem Cell Res. 2020;47:101909 pubmed publisher
Wei L, Zhang J, Chen D, Feng L, Wu C, Wang R, et al. Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi002-A) from a patient with spinocerebellar ataxia type 3. Stem Cell Res. 2020;47:101898 pubmed publisher
Toombs J, Panther L, Ornelas L, Liu C, Gomez E, Martín Ibáñez R, et al. Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936. Stem Cell Res. 2020;46:101851 pubmed publisher
Zhang J, Wei L, Wang R, Li X, Liu Z. Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi001-A) from a healthy male individual. Stem Cell Res. 2020;45:101809 pubmed publisher
Long P, Liu Z, Wu B, Chen J, Sun C, Wang F, et al. Generation of an induced pluripotent stem cell line from chorionic villi of a Patau syndrome spontaneous abortion. Stem Cell Res. 2020;45:101789 pubmed publisher
He L, Zhao H, Li S, Han X, Chen Z, Wang C, et al. Generation of induced pluripotent stem cell line (CSUXHi002-A) from a patient with spinocerebellar ataxia type 1. Stem Cell Res. 2020;45:101816 pubmed publisher
Zhang J, Wei L, Chen D, Feng L, Wu C, Wang R, et al. Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi004-A) from a Wilson's disease patient harboring a homozygous Pro992Leu mutation in ATP7B gene. Stem Cell Res. 2020;44:101741 pubmed publisher
Tanaka A, Watanabe A, Nakano Y, Matsumoto M, Okazaki Y, Miyajima A. Reversible expansion of pancreatic islet progenitors derived from human induced pluripotent stem cells. Genes Cells. 2020;25:302-311 pubmed publisher
Rahman M, Wruck W, Spitzhorn L, Nguyen L, Bohndorf M, Martins S, et al. The FGF, TGFβ and WNT axis Modulate Self-renewal of Human SIX2+ Urine Derived Renal Progenitor Cells. Sci Rep. 2020;10:739 pubmed publisher
Zhang J, Wei L, Chen D, Feng L, Wu C, Wang R, et al. Generation of an integration-free induced pluripotent stem cell (iPSC) line (ZZUNEUi003-A) from a Wilson's disease patient harboring a homozygous R778L mutation in ATP7B gene. Stem Cell Res. 2019;41:101664 pubmed publisher
Georges P, Boza Morán M, Gide J, Peche G, Foret B, Bayot A, et al. Induced pluripotent stem cells-derived neurons from patients with Friedreich ataxia exhibit differential sensitivity to resveratrol and nicotinamide. Sci Rep. 2019;9:14568 pubmed publisher
Khoshchehreh R, Totonchi M, Carlos Ramírez J, Torres R, Baharvand H, Aicher A, et al. Epigenetic reprogramming of primary pancreatic cancer cells counteracts their in vivo tumourigenicity. Oncogene. 2019;38:6226-6239 pubmed publisher
Alimonti J, Ribecco Lutkiewicz M, Sodja C, Jezierski A, Stanimirovic D, Liu Q, et al. Zika virus crosses an in vitro human blood brain barrier model. Fluids Barriers CNS. 2018;15:15 pubmed publisher
Howden S, Thomson J, Little M. Simultaneous reprogramming and gene editing of human fibroblasts. Nat Protoc. 2018;13:875-898 pubmed publisher
Ribecco Lutkiewicz M, Sodja C, Haukenfrers J, Haqqani A, Ly D, Zachar P, et al. A novel human induced pluripotent stem cell blood-brain barrier model: Applicability to study antibody-triggered receptor-mediated transcytosis. Sci Rep. 2018;8:1873 pubmed publisher
Lin C, Liu C, Zhang L, Huang Z, Zhao P, Chen R, et al. Interaction of iPSC-derived neural stem cells on poly(L-lactic acid) nanofibrous scaffolds for possible use in neural tissue engineering. Int J Mol Med. 2018;41:697-708 pubmed publisher
Cheng L, Lei Q, Yin C, Wang H, Jin K, Xiang M. Generation of Urine Cell-Derived Non-integrative Human iPSCs and iNSCs: A Step-by-Step Optimized Protocol. Front Mol Neurosci. 2017;10:348 pubmed publisher
Slamecka J, McClellan S, Wilk A, Laurini J, Manci E, Hoerstrup S, et al. Induced pluripotent stem cells derived from human amnion in chemically defined conditions. Cell Cycle. 2018;17:330-347 pubmed publisher
Ghouzzi V, Bianchi F, Molineris I, Mounce B, Berto G, Rak M, et al. ZIKA virus elicits P53 activation and genotoxic stress in human neural progenitors similar to mutations involved in severe forms of genetic microcephaly. Cell Death Dis. 2016;7:e2440 pubmed publisher
Megges M, Oreffo R, Adjaye J. Episomal plasmid-based generation of induced pluripotent stem cells from fetal femur-derived human mesenchymal stromal cells. Stem Cell Res. 2016;16:128-32 pubmed publisher
Matz P, Adjaye J. Episomal-based generation of an iPS cell line from human fetal foreskin fibroblasts. Stem Cell Res. 2016;16:67-9 pubmed publisher
Howard Jones R, Cheung O, Glen A, Allen N, Stephens P. Integration-Free Reprogramming of Lamina Propria Progenitor Cells. J Dent Res. 2016;95:882-8 pubmed publisher
Matz P, Adjaye J. Generation of iPSC line epiHUVEC from human umbilical vein endothelial cells. Stem Cell Res. 2015;15:581-3 pubmed publisher
Slamecka J, Salimova L, McClellan S, van Kelle M, Kehl D, Laurini J, et al. Non-integrating episomal plasmid-based reprogramming of human amniotic fluid stem cells into induced pluripotent stem cells in chemically defined conditions. Cell Cycle. 2016;15:234-49 pubmed publisher
Si Tayeb K, Idriss S, Champon B, Caillaud A, Pichelin M, Arnaud L, et al. Urine-sample-derived human induced pluripotent stem cells as a model to study PCSK9-mediated autosomal dominant hypercholesterolemia. Dis Model Mech. 2016;9:81-90 pubmed publisher
Jouni M, Si Tayeb K, Es Salah Lamoureux Z, Latypova X, Champon B, Caillaud A, et al. Toward Personalized Medicine: Using Cardiomyocytes Differentiated From Urine-Derived Pluripotent Stem Cells to Recapitulate Electrophysiological Characteristics of Type 2 Long QT Syndrome. J Am Heart Assoc. 2015;4:e002159 pubmed publisher
Kim S, Oceguera Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, et al. KLF4 N-terminal variance modulates induced reprogramming to pluripotency. Stem Cell Reports. 2015;4:727-43 pubmed publisher
Wen Z, Nguyen H, Guo Z, Lalli M, Wang X, Su Y, et al. Synaptic dysregulation in a human iPS cell model of mental disorders. Nature. 2014;515:414-8 pubmed publisher
Goh P, Caxaria S, Casper C, Rosales C, Warner T, Coffey P, et al. A systematic evaluation of integration free reprogramming methods for deriving clinically relevant patient specific induced pluripotent stem (iPS) cells. PLoS ONE. 2013;8:e81622 pubmed publisher
Briggs J, Sun J, Shepherd J, Ovchinnikov D, Chung T, Nayler S, et al. Integration-free induced pluripotent stem cells model genetic and neural developmental features of down syndrome etiology. Stem Cells. 2013;31:467-78 pubmed publisher
Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, et al. An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells. Stem Cells. 2013;31:458-66 pubmed publisher
Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, et al. A more efficient method to generate integration-free human iPS cells. Nat Methods. 2011;8:409-12 pubmed publisher
Yu J, Hu K, Smuga Otto K, Tian S, Stewart R, Slukvin I, et al. Human induced pluripotent stem cells free of vector and transgene sequences. Science. 2009;324:797-801 pubmed publisher
product information
Catalog Number :
20927
Product Name :
pEP4 E02S ET2K
article :
doi10.1126/science.1172482
id2535
pubmed_id19325077
bacterial resistance :
Ampicillin
cloning :
backbonepCEP4
backbone_mutation
backbone_originInvitrogen
backbone_size10600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Episomal/EBNA
growth notes :
Please note Addgene Quality Control sequence found mutation K278T in KLF4. Oct4 and Sox2 are coexpressed under a strong EF promoter. SV40LT and Klf4 are coexpressed under a separate (but identical) EF promoter. This plasmid contains EBNA-1 and OriP for limited maintenance in mammalian cells. These plasmids were used at concentrations of >1.5 mg/ml. When preparing these plasmids for transfection, 3 low concentration maxipreps are done and the elution is combined in about 700 ul. Addgene recommends screening 3-5 colonies immediately upon receipt, as this plasmid may be prone to recombination.
growth strain :
used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; SV40LT and Klf4
growth temp :
Top10 Extremely low copy number
inserts :
Top10F'
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3multiple
cloning_site_5multiple
promoter
sequencing_primer_3
sequencing_primer_5na
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesANOP3, MCOPS3
geneSOX2
id6657
aliasesOCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
genePOU5F1
id5460
aliasesEZF, GKLF
geneKLF4
id9314
genbank_ids
NM_002701
NM_004235.5
NM_003106
mutation
nameOct4 and Sox2; SV40LT and Klf4
shRNA_sequence
size6900
species
9606
Homo sapiens
tags
resistance markers :
523
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA