This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCEP4-M2L
catalog :
20926
citations: 17
Reference
Pourtoy Brasselet S, Sciauvaud A, Boza Morán M, Cailleret M, Jarrige M, Polvèche H, et al. Human iPSC-derived neurons reveal early developmental alteration of neurite outgrowth in the late-occurring neurodegenerative Wolfram syndrome. Am J Hum Genet. 2021;108:2171-2185 pubmed publisher
Jiao H, Walczak B, Lee M, Lemieux M, Li W. GATA6 regulates aging of human mesenchymal stem/stromal cells. Stem Cells. 2021;39:62-77 pubmed publisher
Franz M, Hagenau L, Koch R, Neubauer S, Nowack B, Tzvetkova A, et al. Generation of an iPSC line (UMGWi001-B) from a patient with Floating-Harbor Syndrome (FLHS) carrying a heterozygous SRCAP mutation (p.Arg2444). Stem Cell Res. 2020;49:102028 pubmed publisher
Georges P, Boza Morán M, Gide J, Peche G, Foret B, Bayot A, et al. Induced pluripotent stem cells-derived neurons from patients with Friedreich ataxia exhibit differential sensitivity to resveratrol and nicotinamide. Sci Rep. 2019;9:14568 pubmed publisher
Khoshchehreh R, Totonchi M, Carlos Ramírez J, Torres R, Baharvand H, Aicher A, et al. Epigenetic reprogramming of primary pancreatic cancer cells counteracts their in vivo tumourigenicity. Oncogene. 2019;38:6226-6239 pubmed publisher
Alimonti J, Ribecco Lutkiewicz M, Sodja C, Jezierski A, Stanimirovic D, Liu Q, et al. Zika virus crosses an in vitro human blood brain barrier model. Fluids Barriers CNS. 2018;15:15 pubmed publisher
Ribecco Lutkiewicz M, Sodja C, Haukenfrers J, Haqqani A, Ly D, Zachar P, et al. A novel human induced pluripotent stem cell blood-brain barrier model: Applicability to study antibody-triggered receptor-mediated transcytosis. Sci Rep. 2018;8:1873 pubmed publisher
Cheng L, Lei Q, Yin C, Wang H, Jin K, Xiang M. Generation of Urine Cell-Derived Non-integrative Human iPSCs and iNSCs: A Step-by-Step Optimized Protocol. Front Mol Neurosci. 2017;10:348 pubmed publisher
Slamecka J, McClellan S, Wilk A, Laurini J, Manci E, Hoerstrup S, et al. Induced pluripotent stem cells derived from human amnion in chemically defined conditions. Cell Cycle. 2018;17:330-347 pubmed publisher
Ghouzzi V, Bianchi F, Molineris I, Mounce B, Berto G, Rak M, et al. ZIKA virus elicits P53 activation and genotoxic stress in human neural progenitors similar to mutations involved in severe forms of genetic microcephaly. Cell Death Dis. 2016;7:e2440 pubmed publisher
Megges M, Oreffo R, Adjaye J. Episomal plasmid-based generation of induced pluripotent stem cells from fetal femur-derived human mesenchymal stromal cells. Stem Cell Res. 2016;16:128-32 pubmed publisher
Matz P, Adjaye J. Episomal-based generation of an iPS cell line from human fetal foreskin fibroblasts. Stem Cell Res. 2016;16:67-9 pubmed publisher
Matz P, Adjaye J. Generation of iPSC line epiHUVEC from human umbilical vein endothelial cells. Stem Cell Res. 2015;15:581-3 pubmed publisher
Slamecka J, Salimova L, McClellan S, van Kelle M, Kehl D, Laurini J, et al. Non-integrating episomal plasmid-based reprogramming of human amniotic fluid stem cells into induced pluripotent stem cells in chemically defined conditions. Cell Cycle. 2016;15:234-49 pubmed publisher
Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, et al. An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells. Stem Cells. 2013;31:458-66 pubmed publisher
Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, et al. A more efficient method to generate integration-free human iPS cells. Nat Methods. 2011;8:409-12 pubmed publisher
Yu J, Hu K, Smuga Otto K, Tian S, Stewart R, Slukvin I, et al. Human induced pluripotent stem cells free of vector and transgene sequences. Science. 2009;324:797-801 pubmed publisher
product information
Catalog Number :
20926
Product Name :
pCEP4-M2L
article :
doi10.1126/science.1172482
id2535
pubmed_id19325077
bacterial resistance :
Ampicillin
cloning :
backbonepCEP4
backbone_mutation
backbone_originInvitrogen
backbone_size10200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Episomal/EBNA
growth notes :
Myc and Lin28 are coexpressed under a strong CMV promoter. This plasmid contains EBNA-1 and OriP for limited maintenance in mammalian cells. These plasmids were used at concentrations of >1.5 mg/ml. When preparing these plasmids for transfection, 3 low concentration maxipreps are done and the elution is combined in about 700 ul.
growth strain :
used in the derivation of human iPS cells using non-integrating episomal vectors; expresses cMyc and Lin28
growth temp :
Extremely low copy number
origin :
37
pi :
alt_names
bHLEHE39
c-Myc
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5NheI
promoterCMV
sequencing_primer_3IRES-R2 (gacggcaatatggtggaaa)
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMRTL, MYCC, bHLHe39, c-Myc
geneMYC
id4609
genbank_ids
NM_002467.4
mutation
nameMYC
shRNA_sequence
size1300
species
9606
Homo sapiens
tags
alt_names
CSDD1
FLJ12457
LIN-28
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5Unknown
promoterIRES
sequencing_primer_3EBV_rev
sequencing_primer_5IRES-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCSDD1, LIN-28, LIN28, ZCCHC1, lin-28A
geneLIN28A
id79727
genbank_ids
NM_024674.4
mutation
nameLIN28
shRNA_sequence
size600
species
9606
Homo sapiens
tags
resistance markers :
523
tags :
Low Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA