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product summary
company name :
Addgene
product type :
cDNA
product name :
pEP4 E02S EN2K
catalog :
20925
citations: 20
Reference
Qiu S, Zhang X, Zhang L, Liu Z, Wang L, Jin Z, et al. Generation of the induced pluripotent stem cell line SFMUi001-A from a patient with usher syndrome type 2 caused by biallelic variants in the USH2A gene. Stem Cell Res. 2023;69:103101 pubmed publisher
Pourtoy Brasselet S, Sciauvaud A, Boza Morán M, Cailleret M, Jarrige M, Polvèche H, et al. Human iPSC-derived neurons reveal early developmental alteration of neurite outgrowth in the late-occurring neurodegenerative Wolfram syndrome. Am J Hum Genet. 2021;108:2171-2185 pubmed publisher
Jiao H, Walczak B, Lee M, Lemieux M, Li W. GATA6 regulates aging of human mesenchymal stem/stromal cells. Stem Cells. 2021;39:62-77 pubmed publisher
Georges P, Boza Morán M, Gide J, Peche G, Foret B, Bayot A, et al. Induced pluripotent stem cells-derived neurons from patients with Friedreich ataxia exhibit differential sensitivity to resveratrol and nicotinamide. Sci Rep. 2019;9:14568 pubmed publisher
Khoshchehreh R, Totonchi M, Carlos Ramírez J, Torres R, Baharvand H, Aicher A, et al. Epigenetic reprogramming of primary pancreatic cancer cells counteracts their in vivo tumourigenicity. Oncogene. 2019;38:6226-6239 pubmed publisher
Alimonti J, Ribecco Lutkiewicz M, Sodja C, Jezierski A, Stanimirovic D, Liu Q, et al. Zika virus crosses an in vitro human blood brain barrier model. Fluids Barriers CNS. 2018;15:15 pubmed publisher
Ribecco Lutkiewicz M, Sodja C, Haukenfrers J, Haqqani A, Ly D, Zachar P, et al. A novel human induced pluripotent stem cell blood-brain barrier model: Applicability to study antibody-triggered receptor-mediated transcytosis. Sci Rep. 2018;8:1873 pubmed publisher
Slamecka J, McClellan S, Wilk A, Laurini J, Manci E, Hoerstrup S, et al. Induced pluripotent stem cells derived from human amnion in chemically defined conditions. Cell Cycle. 2018;17:330-347 pubmed publisher
Talbot N, Sparks W, Phillips C, Ealy A, Powell A, Caperna T, et al. Bovine trophectoderm cells induced from bovine fibroblasts with induced pluripotent stem cell reprogramming factors. Mol Reprod Dev. 2017;84:468-485 pubmed publisher
Ghouzzi V, Bianchi F, Molineris I, Mounce B, Berto G, Rak M, et al. ZIKA virus elicits P53 activation and genotoxic stress in human neural progenitors similar to mutations involved in severe forms of genetic microcephaly. Cell Death Dis. 2016;7:e2440 pubmed publisher
Megges M, Oreffo R, Adjaye J. Episomal plasmid-based generation of induced pluripotent stem cells from fetal femur-derived human mesenchymal stromal cells. Stem Cell Res. 2016;16:128-32 pubmed publisher
Matz P, Adjaye J. Episomal-based generation of an iPS cell line from human fetal foreskin fibroblasts. Stem Cell Res. 2016;16:67-9 pubmed publisher
Matz P, Adjaye J. Generation of iPSC line epiHUVEC from human umbilical vein endothelial cells. Stem Cell Res. 2015;15:581-3 pubmed publisher
Slamecka J, Salimova L, McClellan S, van Kelle M, Kehl D, Laurini J, et al. Non-integrating episomal plasmid-based reprogramming of human amniotic fluid stem cells into induced pluripotent stem cells in chemically defined conditions. Cell Cycle. 2016;15:234-49 pubmed publisher
Si Tayeb K, Idriss S, Champon B, Caillaud A, Pichelin M, Arnaud L, et al. Urine-sample-derived human induced pluripotent stem cells as a model to study PCSK9-mediated autosomal dominant hypercholesterolemia. Dis Model Mech. 2016;9:81-90 pubmed publisher
Jouni M, Si Tayeb K, Es Salah Lamoureux Z, Latypova X, Champon B, Caillaud A, et al. Toward Personalized Medicine: Using Cardiomyocytes Differentiated From Urine-Derived Pluripotent Stem Cells to Recapitulate Electrophysiological Characteristics of Type 2 Long QT Syndrome. J Am Heart Assoc. 2015;4:e002159 pubmed publisher
Kim S, Oceguera Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, et al. KLF4 N-terminal variance modulates induced reprogramming to pluripotency. Stem Cell Reports. 2015;4:727-43 pubmed publisher
Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, et al. An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells. Stem Cells. 2013;31:458-66 pubmed publisher
Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, et al. A more efficient method to generate integration-free human iPS cells. Nat Methods. 2011;8:409-12 pubmed publisher
Yu J, Hu K, Smuga Otto K, Tian S, Stewart R, Slukvin I, et al. Human induced pluripotent stem cells free of vector and transgene sequences. Science. 2009;324:797-801 pubmed publisher
product information
Catalog Number :
20925
Product Name :
pEP4 E02S EN2K
article :
doi10.1126/science.1172482
id2535
pubmed_id19325077
bacterial resistance :
Ampicillin
cloning :
backbonepCEP4
backbone_mutation
backbone_originInvitrogen
backbone_size10500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Episomal/EBNA
growth notes :
Oct4 and Sox2 are coexpressed under a strong EF promoter. Nanog and Klf4 are coexpressed under a separate (but identical) EF promoter. This plasmid contains EBNA-1 and OriP for limited maintenance in mammalian cells. These plasmids were used at concentrations of >1.5 mg/ml. When preparing these plasmids for transfection, 3 low concentration maxipreps are done and the elution is combined in about 700 ul.
growth strain :
used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Klf4
growth temp :
Top10 Extremely low copy number
inserts :
Top10F'
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3multiple
cloning_site_5multiple
promoter
sequencing_primer_3
sequencing_primer_5na
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesANOP3, MCOPS3
geneSOX2
id6657
aliasesOCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
genePOU5F1
id5460
aliases
geneNANOG
id79923
aliasesEZF, GKLF
geneKLF4
id9314
genbank_ids
NM_002701
NM_004235
NM_024865
NM_003106
mutation
nameOct4 and Sox2; Nanog and Klf4
shRNA_sequence
size5700
species
9606
Homo sapiens
tags
resistance markers :
523
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA