pEP4 E02S CK2M EN2L | Addgene 20924 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEP4 E02S CK2M EN2L

catalog :

20924

citations: 15

Reference
Singh A, Singh S, Kumar M, Sarma D, Singh S, Verma V. Establishment of Capra hircus somatic cells and induction of pluripotent stem-like cells. In Vitro Cell Dev Biol Anim. 2023;: pubmed publisher
Rahman M, Wruck W, Spitzhorn L, Nguyen L, Bohndorf M, Martins S, et al. The FGF, TGFβ and WNT axis Modulate Self-renewal of Human SIX2+ Urine Derived Renal Progenitor Cells. Sci Rep. 2020;10:739 pubmed publisher
Li S, Zheng E, Zhao L, Liu S. Nonreciprocal and Conditional Cooperativity Directs the Pioneer Activity of Pluripotency Transcription Factors. Cell Rep. 2019;28:2689-2703.e4 pubmed publisher
Khoshchehreh R, Totonchi M, Carlos Ramírez J, Torres R, Baharvand H, Aicher A, et al. Epigenetic reprogramming of primary pancreatic cancer cells counteracts their in vivo tumourigenicity. Oncogene. 2019;38:6226-6239 pubmed publisher
Moro L, Amin G, Furmento V, Waisman A, Garate X, Neiman G, et al. MicroRNA characterization in equine induced pluripotent stem cells. PLoS ONE. 2018;13:e0207074 pubmed publisher
Howard Jones R, Cheung O, Glen A, Allen N, Stephens P. Integration-Free Reprogramming of Lamina Propria Progenitor Cells. J Dent Res. 2016;95:882-8 pubmed publisher
Si Tayeb K, Idriss S, Champon B, Caillaud A, Pichelin M, Arnaud L, et al. Urine-sample-derived human induced pluripotent stem cells as a model to study PCSK9-mediated autosomal dominant hypercholesterolemia. Dis Model Mech. 2016;9:81-90 pubmed publisher
Jouni M, Si Tayeb K, Es Salah Lamoureux Z, Latypova X, Champon B, Caillaud A, et al. Toward Personalized Medicine: Using Cardiomyocytes Differentiated From Urine-Derived Pluripotent Stem Cells to Recapitulate Electrophysiological Characteristics of Type 2 Long QT Syndrome. J Am Heart Assoc. 2015;4:e002159 pubmed publisher
Miyagawa Y, Marino P, Verlengia G, Uchida H, Goins W, Yokota S, et al. Herpes simplex viral-vector design for efficient transduction of nonneuronal cells without cytotoxicity. Proc Natl Acad Sci U S A. 2015;112:E1632-41 pubmed publisher
Kim S, Oceguera Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, et al. KLF4 N-terminal variance modulates induced reprogramming to pluripotency. Stem Cell Reports. 2015;4:727-43 pubmed publisher
Goh P, Caxaria S, Casper C, Rosales C, Warner T, Coffey P, et al. A systematic evaluation of integration free reprogramming methods for deriving clinically relevant patient specific induced pluripotent stem (iPS) cells. PLoS ONE. 2013;8:e81622 pubmed publisher
Briggs J, Sun J, Shepherd J, Ovchinnikov D, Chung T, Nayler S, et al. Integration-free induced pluripotent stem cells model genetic and neural developmental features of down syndrome etiology. Stem Cells. 2013;31:467-78 pubmed publisher
Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, et al. An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells. Stem Cells. 2013;31:458-66 pubmed publisher
Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, et al. A more efficient method to generate integration-free human iPS cells. Nat Methods. 2011;8:409-12 pubmed publisher
Yu J, Hu K, Smuga Otto K, Tian S, Stewart R, Slukvin I, et al. Human induced pluripotent stem cells free of vector and transgene sequences. Science. 2009;324:797-801 pubmed publisher

product information

Catalog Number :

20924

Product Name :

pEP4 E02S CK2M EN2L

article :

doi	10.1126/science.1172482
id	2535
pubmed_id	19325077

bacterial resistance :

Ampicillin

cloning :

backbone

pCEP4

backbone_mutation

backbone_origin

Invitrogen

backbone_size

11600

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Episomal/EBNA

growth notes :

Oct4 and Sox2 are coexpressed under a strong EF promoter. Nanog and Lin28 are coexpressed under a separate (but identical) EF promoter. Klf4 and Myc are coexpressed under a strong CMV promoter. This plasmid contains EBNA-1 and OriP for limited maintenance in mammalian cells. These plasmids were used at concentrations of >1.5 mg/ml. When preparing these plasmids for transfection, 3 low concentration maxipreps are done and the elution is combined in about 700 ul. Note that there are some point mutations in the Klf4 gene, but they do not effect the function of this plasmid.

growth strain :

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Klf4 and Myc; Nanog and Lin28

growth temp :

Top10 Extremely low copy number

inserts :

Top10F'

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	multiple
cloning_site_5	multiple
promoter
sequencing_primer_3
sequencing_primer_5	na
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MRTL, MYCC, bHLHe39, c-Myc
gene	MYC
id	4609

aliases	ANOP3, MCOPS3
gene	SOX2
id	6657

aliases	EZF, GKLF
gene	KLF4
id	9314

aliases
gene	NANOG
id	79923

aliases	OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
gene	POU5F1
id	5460

aliases	CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A
gene	LIN28A
id	79727

genbank_ids

NM_002701

NM_002467

NM_004235

NM_024674

NM_024865

NM_003106

mutation

name

Oct4 and Sox2; Nanog and Lin28; Klf4 and Myc

shRNA_sequence

size

4900

species

9606

Homo sapiens