pEP4 E02S EM2K | Addgene 20923 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEP4 E02S EM2K

catalog :

20923

citations: 4

Reference
Baik J, Ortiz Cordero C, Magli A, Azzag K, Crist S, Yamashita A, et al. Establishment of Skeletal Myogenic Progenitors from Non-Human Primate Induced Pluripotent Stem Cells. Cells. 2023;12: pubmed publisher
Howden S, Hosseini Far H, Motazedian A, Elefanty A, Stanley E, Lamande S, et al. The use of simultaneous reprogramming and gene correction to generate an osteogenesis imperfecta patient COL1A1 c. 3936 G>T iPSC line and an isogenic control iPSC line. Stem Cell Res. 2019;38:101453 pubmed publisher
Olivera R, Moro L, Jordan R, Luzzani C, Miriuka S, Radrizzani M, et al. In Vitro and In Vivo Development of Horse Cloned Embryos Generated with iPSCs, Mesenchymal Stromal Cells and Fetal or Adult Fibroblasts as Nuclear Donors. PLoS ONE. 2016;11:e0164049 pubmed publisher
Yu J, Hu K, Smuga Otto K, Tian S, Stewart R, Slukvin I, et al. Human induced pluripotent stem cells free of vector and transgene sequences. Science. 2009;324:797-801 pubmed publisher

product information

Catalog Number :

20923

Product Name :

pEP4 E02S EM2K

article :

doi	10.1126/science.1172482
id	2535
pubmed_id	19325077

bacterial resistance :

Ampicillin

cloning :

backbone

pCEP4

backbone_mutation

backbone_origin

Invitrogen

backbone_size

12600

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Episomal/EBNA

growth notes :

Oct4 and Sox2 are coexpressed under a strong EF promoter. Myc and Klf4 are coexpressed under a separate (but identical) EF promoter. This plasmid contains EBNA-1 and OriP for limited maintenance in mammalian cells. These plasmids were used at concentrations of >1.5 mg/ml. When preparing these plasmids for transfection, 3 low concentration maxipreps are done and the elution is combined in about 700 ul. Note that this a K278M mutation in Klf4, but it does not affect the reprogramming ability.

growth strain :

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Myc and Klf4

growth temp :

Top10 Extremely low copy number

inserts :

Top10F'

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	multiple
cloning_site_5	multiple
promoter
sequencing_primer_3
sequencing_primer_5	na
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ANOP3, MCOPS3
gene	SOX2
id	6657

aliases	EZF, GKLF
gene	KLF4
id	9314

aliases	MRTL, MYCC, bHLHe39, c-Myc
gene	MYC
id	4609

aliases	OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
gene	POU5F1
id	5460

genbank_ids

NM_002701

NM_004235

NM_002467

NM_003106

mutation

name

Oct4 and Sox2; Myc and Klf4

shRNA_sequence

size

2700

species

9606

Homo sapiens