pEP4 E02S EN2L | Addgene 20922 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEP4 E02S EN2L

catalog :

20922

citations: 9

Reference
Baik J, Ortiz Cordero C, Magli A, Azzag K, Crist S, Yamashita A, et al. Establishment of Skeletal Myogenic Progenitors from Non-Human Primate Induced Pluripotent Stem Cells. Cells. 2023;12: pubmed publisher
Sharma R, Choi K, Quan M, Sharma S, Sankaran B, Park H, et al. Liquid condensation of reprogramming factor KLF4 with DNA provides a mechanism for chromatin organization. Nat Commun. 2021;12:5579 pubmed publisher
Howden S, Thomson J, Little M. Simultaneous reprogramming and gene editing of human fibroblasts. Nat Protoc. 2018;13:875-898 pubmed publisher
Hu D, Ferro F, Yang F, Taylor A, Chang W, Miclau T, et al. Cartilage to bone transformation during fracture healing is coordinated by the invading vasculature and induction of the core pluripotency genes. Development. 2017;144:221-234 pubmed publisher
Si Tayeb K, Idriss S, Champon B, Caillaud A, Pichelin M, Arnaud L, et al. Urine-sample-derived human induced pluripotent stem cells as a model to study PCSK9-mediated autosomal dominant hypercholesterolemia. Dis Model Mech. 2016;9:81-90 pubmed publisher
Jouni M, Si Tayeb K, Es Salah Lamoureux Z, Latypova X, Champon B, Caillaud A, et al. Toward Personalized Medicine: Using Cardiomyocytes Differentiated From Urine-Derived Pluripotent Stem Cells to Recapitulate Electrophysiological Characteristics of Type 2 Long QT Syndrome. J Am Heart Assoc. 2015;4:e002159 pubmed publisher
Wen Z, Nguyen H, Guo Z, Lalli M, Wang X, Su Y, et al. Synaptic dysregulation in a human iPS cell model of mental disorders. Nature. 2014;515:414-8 pubmed publisher
Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, et al. A more efficient method to generate integration-free human iPS cells. Nat Methods. 2011;8:409-12 pubmed publisher
Yu J, Hu K, Smuga Otto K, Tian S, Stewart R, Slukvin I, et al. Human induced pluripotent stem cells free of vector and transgene sequences. Science. 2009;324:797-801 pubmed publisher

product information

Catalog Number :

20922

Product Name :

pEP4 E02S EN2L

article :

doi	10.1126/science.1172482
id	2535
pubmed_id	19325077

bacterial resistance :

Ampicillin

cloning :

backbone

pCEP4

backbone_mutation

backbone_origin

Invitrogen

backbone_size

10500

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

Episomal/EBNA

growth notes :

Oct4 and Sox2 are coexpressed under a strong EF promoter. Nanog and Lin28 are coexpressed under a separate (but identical) EF promoter. This plasmid contains EBNA-1 and OriP for limited maintenance in mammalian cells. These plasmids were used at concentrations of >1.5 mg/ml. When preparing these plasmids for transfection, 3 low concentration maxipreps are done and the elution is combined in about 700 ul.

growth strain :

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Lin28

growth temp :

Top10 Extremely low copy number

inserts :

Top10F'

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	multiple
cloning_site_5	multiple
promoter
sequencing_primer_3
sequencing_primer_5	na
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
gene	POU5F1
id	5460

aliases	ANOP3, MCOPS3
gene	SOX2
id	6657

aliases
gene	NANOG
id	79923

aliases	CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A
gene	LIN28A
id	79727

genbank_ids

NM_002701

NM_024674

NM_024865

NM_003106

mutation

name

Oct4 and Sox2; Nanog and Lin28

shRNA_sequence

size

4900

species

9606

Homo sapiens