This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pUC-T7-hTP53-P0~670-FLuc
catalog :
208640
product information
Catalog Number :
208640
Product Name :
pUC-T7-hTP53-P0~670-FLuc
article :
doi
id28243088
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepUC19
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Luciferase
Other
In vitor transcription template
growth notes :
For in vitro transcription, Linearize with HindIII
growth strain :
for in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.
origin :
37
pi :
alt_names
P53-IRES-P0
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3M13-R
sequencing_primer_5M13-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutationUTR and partial CDS in-frame with F-Luc
nameTP53, UTR and partial CDS
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagT7 promoter
locationC terminal on insert
tagFirefly Luciferase (F-Luc)
plasmid copy :
F-luc was cloned from pGL3-Basic.
resistance markers :
6738
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA