This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET28-eIF4E-TwStrep
catalog :
208638
product information
Catalog Number :
208638
Product Name :
pET28-eIF4E-TwStrep
article :
doi
id28243088
pubmed_id
bacterial resistance :
Kanamycin
cloning :
backbonepET28-3'TwStrep
backbone_mutationrefer to (Addgene#208053)
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
for E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)
growth temp :
we use BL21 (DE3) for expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5NdeI
promoterT7
sequencing_primer_3T7ter
sequencing_primer_5pETupstream, T7p
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAUTS19, CBP, EIF4E1, EIF4EL1, EIF4F, eIF-4E
geneEIF4E
id1977
genbank_ids
ENST00000450253
mutation
namehuman eIF4E (ENST00000450253)
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagTwin Strep
locationN terminal on backbone
tag6xHis
resistance markers :
6738
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA