This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET28-Flag-RBM24-95~236
catalog :
208634
product information
Catalog Number :
208634
Product Name :
pET28-Flag-RBM24-95~236
article :
doi
id28243088
pubmed_id
bacterial resistance :
Kanamycin
cloning :
backbonepET28a(+)
backbone_mutation
backbone_originMerck-Millipore (Novagen)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
#important# For this plasmid, we failed to obtain enough soluble protein with standard protocol using BL21(DE3) and Ni-NTA. Need further optimization.
growth strain :
for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236
origin :
37
pi :
alt_names
RBM24
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3T7ter
sequencing_primer_5pETupstream, T7p
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesRNPC6, dJ259A10.1
geneRBM24
id221662
genbank_ids
ENST00000379052
mutationonly aa 95~236, other residues not included
namehuman RBM24 (ENST00000216146) aa 95~236
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
locationN terminal on backbone
tag6xHis
resistance markers :
6738
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA