p11d-aRPA (MSW#712) | Addgene 208078 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

p11d-aRPA (MSW#712)

catalog :

208078

citations: 1

Reference
Mason A, Haring S, Pryor J, Staloch C, Gan T, Wold M. An alternative form of replication protein a prevents viral replication in vitro. J Biol Chem. 2009;284:5324-31 pubmed publisher

product information

Catalog Number :

208078

Product Name :

p11d-aRPA (MSW#712)

article :

doi	10.1074/jbc.M808963200
id	28243095
pubmed_id	19116208

bacterial resistance :

Ampicillin

cloning :

backbone

pET11d

backbone_mutation

backbone_origin

MilliporeSigma

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Contains a single T7 RNA polymerase promoter followed by RPA70, RPA14 and RPA4. (Each gene has Shine-Delgarno ribosome binding site.) Induction with IPTGexpresses all three subunits to produce the Alternative RPA complex (Alt-RPA).

growth strain :

Expression of Alternative human Replication Protein A (RPA1,4,3) in E. coli

origin :

pi :

alt_names

RPA70, RPA4, RPA14

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5	Unknown
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HSSB, MST075, PFBMFT6, REPA1, RF-A, RP-A, RPA70
gene	RPA1
id	6117

aliases	REPA3, RP-A p14
gene	RPA3
id	6119

aliases	HSU24186
gene	RPA4
id	29935

genbank_ids

mutation

name

RPA1, RPA4, RPA3

shRNA_sequence

size

species

9606

Homo sapiens