p11d-tRPA (MSW#111) | Addgene 208066 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

p11d-tRPA (MSW#111)

catalog :

208066

product information

Catalog Number :

208066

Product Name :

p11d-tRPA (MSW#111)

article :

doi
id	28191952
pubmed_id	8157639

bacterial resistance :

Ampicillin

cloning :

backbone

pET11d

backbone_mutation

backbone_origin

MilliporeSigma

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Contains a single T7 RNA polymerase promoter followed by RPA70, RPA14 and RPA32. (Each gene has Shine-Delgarno ribosome binding site.) Induction with IPTGexpresses RPA70, RPA14 and RPA32 which form reconbinant RPA complex.

growth strain :

expression of human Replication Protein A in E. coli

origin :

pi :

alt_names

RPA70, RPA32, RPA14

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5	Unknown
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HSSB, MST075, PFBMFT6, REPA1, RF-A, RP-A, RPA70
gene	RPA1
id	6117

aliases	REPA3, RP-A p14
gene	RPA3
id	6119

aliases	REPA2, RP-A p32, RP-A p34, RPA32
gene	RPA2
id	6118

genbank_ids

mutation

RPA32 has a point mutation Thr88(ACC) -> Ile(ATC)

name

RPA1, RPA2, RPA3

shRNA_sequence

size

species

9606

Homo sapiens