TRIPZ-bioGEF-SUMO1nc-PURO | Addgene 208042 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

TRIPZ-bioGEF-SUMO1nc-PURO

catalog :

208042

citations: 1

Reference
Barroso Gomila O, Merino Cacho L, Muratore V, Pérez C, Taibi V, Maspero E, et al. BioE3 identifies specific substrates of ubiquitin E3 ligases. Nat Commun. 2023;14:7656 pubmed publisher

product information

Catalog Number :

208042

Product Name :

TRIPZ-bioGEF-SUMO1nc-PURO

article :

doi	10.1038/s41467-023-43326-8
id	28243085
pubmed_id	37996419

bacterial resistance :

Ampicillin

cloning :

backbone

TRIPZ

backbone_mutation

backbone_origin

Open Biosystems

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Lentiviral

growth notes :

Lentiviral packaging of TRIPZ is more efficient with TAT expression (e.g using pcDNA1-TatHIV Addgene ID: 138478) and 2nd generation gag/pol/env combination (e.g. using PSPAX2 Addgene ID: 12260 and pMD2.G Addgene ID: 12259); Use of bioGEF-UBwt may be necessary for certain E3 ligases (e.g. NEDD4 HECT ligase); Use of non-cleavable versions may reduce recycling of biotinylated UB and may lead to higher yields of biotinylated protein for proteomic analysis, but induction times should be minimized to avoid toxicity.

growth strain :

Enables inducible expression of low-affinity Avitag-SUMO1nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	tetO/UBC
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1
gene	SUMO1
id	7341

genbank_ids

mutation

Q94P Mutation near C-terminus suppresses cleavage by SENPs/DUBs

name

SUMO1nc

shRNA_sequence

size

species

9606

Homo sapiens