TRIPZ-CTID195-GSQ-SUMO1nc-PURO | Addgene 208035 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

TRIPZ-CTID195-GSQ-SUMO1nc-PURO

catalog :

208035

citations: 1

Reference
Barroso Gomila O, Trulsson F, Muratore V, Canosa I, Merino Cacho L, Cortazar A, et al. Identification of proximal SUMO-dependent interactors using SUMO-ID. Nat Commun. 2021;12:6671 pubmed publisher

product information

Catalog Number :

208035

Product Name :

TRIPZ-CTID195-GSQ-SUMO1nc-PURO

article :

doi	10.1038/s41467-021-26807-6
id	28226798
pubmed_id	34795231

bacterial resistance :

Ampicillin

cloning :

backbone

TRIPZ

backbone_mutation

backbone_origin

Open Biosystems

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Lentiviral

growth notes :

Lentiviral packaging of TRIPZ is more efficient with TAT expression (e.g using pcDNA1-TatHIV Addgene ID: 138478) and 2nd generation gag/pol/env combination (e.g. using PSPAX2 Addgene ID: 12260 and pMD2.G Addgene ID: 12259); SUMO1nc is excisable using EcoR1-Mlu1.

growth strain :

Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	tetO/UBC
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	DAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1
gene	SUMO1
id	7341

genbank_ids

mutation

Q94P Mutation near C-terminus suppresses cleavage by SENPs/DUBs

name

SUMO1nc

shRNA_sequence

size

species

9606

Homo sapiens