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product summary
company name :
Addgene
product type :
cDNA
product name :
miR-206-1/miR-133b promoter GFP
catalog :
20793
citations: 1
product information
Catalog Number :
20793
Product Name :
miR-206-1/miR-133b promoter GFP
article :
| doi | 10.1101/gad.1745709 |
| id | 2508 |
| pubmed_id | 19240133 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pBSII SK+ modified to introduce GFP and more cloning sites | ||
| backbone_mutation | |||
| backbone_origin | Bartel Lab | ||
| backbone_size | |||
| promoter | |||
| sequencing_primer_3 | |||
| sequencing_primer_5 | |||
| vector_types |
|
growth notes :
For polycistronic mir-206-1/mir-133b, we were not able to identify the transcriptional start precisely by 5 -RACE due to the repetitive sequence in this region. Analyses of EST clones (BM259633, CF999541, CF348604, DR729161) mapped the approximate start of the mir-206-1/mir-133b primary transcript to 1.4 kb from the mir-206 hairpin. A 4.5-kb upstream fragment that includes 0.9 kb of the primary transcript was amplified from genomic DNA using primers 3 and 4 (5'-CTGCCCCATTTAGACTCTTTCTTGACG-3' and 5'-CCAGAAGAACCCAGACGAAACCCAC-3'). The promoter fragment was fused to a GFP reporter gene. The miRNA promoter fusion was flanked by I-SceI meganuclease recognition sites to increase the efficiency of transgenensis. Please note that Addgene's sequencing results show some differences from the published sequence. The depositing lab is aware of the differences.
origin :
37
pi :
|
resistance markers :
| 21 |
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments