miR-124-5 promoter GFP | Addgene 20792 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

miR-124-5 promoter GFP

catalog :

20792

citations: 1

Reference
Shkumatava A, Stark A, Sive H, Bartel D. Coherent but overlapping expression of microRNAs and their targets during vertebrate development. Genes Dev. 2009;23:466-81 pubmed publisher

product information

Catalog Number :

20792

Product Name :

miR-124-5 promoter GFP

article :

doi	10.1101/gad.1745709
id	2508
pubmed_id	19240133

bacterial resistance :

Ampicillin

cloning :

backbone

pBSII SK+ modified to introduce GFP and more cloning sites

backbone_mutation

backbone_origin

Bartel Lab

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

zebrafish expression

growth notes :

5 -RACE (GenRacer kit, Invitrogen) identified a transcriptional start of the mir-124-5 primary transcript, which is located 1.1 kb from the mir-124-5 hairpin. A 5.6-kb fragment upstream of the mir-124-5 transcriptional start was amplified from genomic DNA using primers 1 and 2 (5'-TGCTTTGAGGCACGGTTATGGTCCC-3' and 5'-CTCGGGCAGCGGCTTTTTGGTCCTG-3'). The promoter fragment was fused to a GFP reporter gene. The miRNA promoter fusion was flanked by I-SceI meganuclease recognition sites to increase the efficiency of transgenensis.

origin :

pi :

alt_names

miR124

cloning

clone_method	Restriction Enzyme
cloning_site_3	SalI
cloning_site_5	SmaI
promoter
sequencing_primer_3
sequencing_primer_5	TGCTTTGAGGCACGGTTATGGTCCC
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases	dre-mir-124-5
gene	mir124-5
id	100033632

genbank_ids

mutation

name

mir-124

shRNA_sequence

size

5600

species

7955

Danio rerio